Publication: Up-regulation of Ribophorin I after yellow head virus (YHV) challenge in black tiger shrimp Penaeus monodon
Issued Date
2008-07-01
Resource Type
ISSN
10959947
10504648
10504648
Other identifier(s)
2-s2.0-44749086663
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Fish and Shellfish Immunology. Vol.25, No.1-2 (2008), 40-46
Suggested Citation
Sudkhate Molthathong, Arun Buaklin, Saengchan Senapin, Sirawut Klinbunga, Jiraporn Rojtinnakorn, Timothy W. Flegel Up-regulation of Ribophorin I after yellow head virus (YHV) challenge in black tiger shrimp Penaeus monodon. Fish and Shellfish Immunology. Vol.25, No.1-2 (2008), 40-46. doi:10.1016/j.fsi.2007.12.003 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/18720
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Up-regulation of Ribophorin I after yellow head virus (YHV) challenge in black tiger shrimp Penaeus monodon
Abstract
This work constitutes the second report from a continuing investigation of shrimp genes that may be involved in apoptosis associated death resulting from yellow head virus (YHV) infection. Here, we describe from the black tiger shrimp Penaeus monodon, a ribophorin I-like gene that is probably a subunit of the oligosaccharyltransferase complex (OST), a key enzyme in N-linked glycosylation that occurs in the endoplasmic reticulum. The OST complex also contains DAD1 (defender against apoptotic death 1) that has been reported to control apoptosis and that we have previously reported from P. monodon. The full length ribophorin I of P. monodon comprised 2157 bp with the ORF of 1806 bp corresponding to 601 deduced amino acids and three putative N-linked glycosylation sites. Analysis revealed hydrophobic properties implying that it could be a membrane protein. Tissue distribution analysis using real-time RT-PCR with SYBR Green revealed that ribophorin I was endogenously expressed in all examined tissues of normal shrimp. However, unlike DAD1 that was down-regulated after YHV challenge, ribophorin I expression was up-regulated and remained high until the moribund stage. © 2007 Elsevier Ltd. All rights reserved.