Morus alba leaf extracts prevent indomethacin-induced intestinal ulcers via suppression of endoplasmic reticulum stress
Issued Date
2025-01-01
Resource Type
eISSN
22313354
Scopus ID
2-s2.0-105000132064
Journal Title
Journal of Applied Pharmaceutical Science
Volume
15
Issue
3
Start Page
101
End Page
111
Rights Holder(s)
SCOPUS
Bibliographic Citation
Journal of Applied Pharmaceutical Science Vol.15 No.3 (2025) , 101-111
Suggested Citation
Boonyong C., Angkhasirisap W., Lertnitikul N., Kengkoom K., Suttisri R., Jianmongkol S. Morus alba leaf extracts prevent indomethacin-induced intestinal ulcers via suppression of endoplasmic reticulum stress. Journal of Applied Pharmaceutical Science Vol.15 No.3 (2025) , 101-111. 111. doi:10.7324/JAPS.2025.210080 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/108543
Title
Morus alba leaf extracts prevent indomethacin-induced intestinal ulcers via suppression of endoplasmic reticulum stress
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Abstract
This study investigated the gastrointestinal (GI) protective effect of water and ethanol extracts of Morus alba leaves in rats who received indomethacin. Rats were randomly distributed into nine groups: a control group, an indomethacin group, and seven groups pre-treated orally with either water and ethanol extracts of M. alba leaves (500, 1,000, and 2,000 mg/kg) or omeprazole (100 mg/kg) for 5 days before receiving oral indomethacin (40 mg/kg). After 24-hour treatment, GI lesions, apoptotic proteins, and prostaglandin E2 (PGE2) in gastric and jejunal tissues were evaluated by macro- and microscopic assessments, western blot analysis, and ELISA, respectively. Both extracts prevented ulcerative lesions in rat jejunum, but not in the stomach. Their intestinal protection involved suppressing protein kinase R-like ER kinase/C/EBP homologous protein signaling and the Bcl-2-associated X protein/B-cell leukemia/lymphoma 2 protein (Bax/Bcl-2) ratio. Both extracts did not affect PGE2 levels in rat GI tissues, which might explain their inability to prevent gastric ulcers.