Publication: Rubber formation by fresh bottom fraction of Hevea latex
1
Issued Date
1997-05-01
Resource Type
ISSN
00319422
Other identifier(s)
2-s2.0-0030935368
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Phytochemistry. Vol.45, No.2 (1997), 269-274
Suggested Citation
Jitladda Tangpakdee, Yasuyuki Tanaka, Kyozo Ogura, Tanetoshi Koyama, Rapepun Wititsuwannakul, Dhirayos Wititsuwannakul Rubber formation by fresh bottom fraction of Hevea latex. Phytochemistry. Vol.45, No.2 (1997), 269-274. doi:10.1016/S0031-9422(96)00838-2 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/17861
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Rubber formation by fresh bottom fraction of Hevea latex
Abstract
The formation de novo of rubber was confirmed by the incorporation of14C-IDP into rubber by fresh bottom fraction (BF) and C-serum (CS) at 37°for 6 hr after pre-incubation with the substrate at 4 overnight. The incorporation of14C-IDP and rubber yield were highest in fresh BF, A control experiment with boiled BF showed practically no incorporation of14C-IDP, although it contained about 1% (w/w dried BF) of rubber corresponding to pre-existing rubber. A very small amount of rubber was formed on incubation of CS and14C-IDP. Rubber formation in fresh Bf was inhibited by the addition of NaF. Freeze-dried BF formed no rubber, although it was able to form polyprenols. The radiolabelled rubber formed on incubation of fresh Bf and14C-IDP showed a molecular-weight distribution (MWD) having a peak similar to the low molecular-weight fraction of the skewed unimodal distribution of the endogenous rubber. Membrane-bound particles in fresh BF are presumed to have an important role in the synthesis of new rubber molecules.