Browsing by Author "Ajcharapan Tantipanjaporn"

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    A thiourea-appended rhodamine chemodosimeter for mercury(II) and its bioimaging application
    (2018-03-05) Ajcharapan Tantipanjaporn; Samran Prabpai; Kanoknetr Suksen; Palangpon Kongsaeree; Mahidol University
    © 2017 Elsevier B.V. A rhodamine-thiourea conjugate RTP with an o-phenylenediamine linker was developed as a fluorogenic chemodosimeter for Hg2+ detection. In the presence of Hg2+, a colorless solution of RTP turned pink with a maximum absorption band at 555 nm and with a 62-fold fluorescence enhancement at 578 nm (Φ = 0.34). RTP is highly selective to Hg2+ among other metal ions with a detection limit of 1.6 nM (0.3 ppb). A similar rhodamine analog with a flexible ethylenediamine spacer was less selective and less sensitive than RTP. Hg2+ induced cyclic guanylation to yield a benzimidazole moiety and a subsequent ring-opening of the spirolactam unit resulted in chromogenic and fluorogenic changes. The membrane-permeable RTP probe was successfully demonstrated in monitoring of Hg2+ in cultured HeLa cells.