Browsing by Author "Atithep T."

Filter results by typing the first few letters
Now showing 1 - 4 of 4
  • No Thumbnail Available
    ItemMetadata only
    A novel virulent Litunavirus phage possesses therapeutic value against multidrug resistant Pseudomonas aeruginosa
    (2022-12-01) Lerdsittikul V.; Thongdee M.; Chaiwattanarungruengpaisan S.; Atithep T.; Apiratwarrasakul S.; Withatanung P.; Clokie M.R.J.; Korbsrisate S.; Mahidol University
    Pseudomonas aeruginosa is a notable nosocomial pathogen that can cause severe infections in humans and animals. The emergence of multidrug resistant (MDR) P. aeruginosa has motivated the development of phages to treat the infections. In this study, a novel Pseudomonas phage, vB_PaeS_VL1 (VL1), was isolated from urban sewage. Phylogenetic analyses revealed that VL1 is a novel species in the genus Litunavirus of subfamily Migulavirinae. The VL1 is a virulent phage as no genes encoding lysogeny, toxins or antibiotic resistance were identified. The therapeutic potential of phage VL1 was investigated and revealed that approximately 56% (34/60 strains) of MDR P. aeruginosa strains, isolated from companion animal diseases, could be lysed by VL1. In contrast, VL1 did not lyse other Gram-negative and Gram-positive bacteria suggesting its specificity of infection. Phage VL1 demonstrated high efficiency to reduce bacterial load (~ 6 log cell number reduction) and ~ 75% reduction of biofilm in pre-formed biofilms of MDR P. aeruginosa. The result of two of the three MDR P. aeruginosa infected Galleria mellonella larvae showed that VL1 could significantly increase the survival rate of infected larvae. Taken together, phage VL1 has genetic and biological properties that make it a potential candidate for phage therapy against P. aeruginosa infections.
  • No Thumbnail Available
    ItemMetadata only
    Isolation and characterisation of a novel Silviavirus bacteriophage promising antimicrobial agent against methicillin-resistant Staphylococcus aureus infections
    (2024-12-01) Lerdsittikul V.; Apiratwarrasakul S.; Atithep T.; Withatanung P.; Indrawattana N.; Pumirat P.; Chaiwattanarungruengpaisan S.; Thongdee M.; Lerdsittikul V.; Mahidol University
    The increasing prevalence of methicillin-resistant Staphylococcus aureus (MRSA) emphasises the urgent need for novel antimicrobial agents as alternatives to antibiotics. Bacteriophage therapy is one of the most promising antimicrobial strategies. Here, we isolated and comprehensively characterized a novel Staphylococcus phage, vB_SauM_VL10 (VL10), from urban sewage. The VL10 genome displays 141,746 bp of linear double-stranded DNA, containing 193 open reading frames and lacking tRNA, virulence, or antibiotic resistance genes. Phylogenetic analysis categorizes VL10 as a novel species within the Silviavirus genus, Twortvirinae subfamily. VL10 exhibits lytic behaviour characterized by efficient adsorption, a short latent period, and substantial burst size, with environmental stability. It demonstrates lytic activity against 79.06% of tested S. aureus strains, highlighting its species specificity. Additionally, VL10 effectively targets MRSA biofilms, reducing biomass and viable cells. In MRSA-infected G. mellonella larvae, VL10 enhances survival rates, supporting its potential for phage therapy applications. Moreover, the emergence of VL10-resistant S. aureus strains associated with fitness trade-offs, including reduced growth, biofilm formation, and virulence. Altogether, these findings emphasize VL10 as a promising candidate for developing therapeutic agents against MRSA infections, providing insights into phage biology and resistance dynamics.
  • No Thumbnail Available
    ItemMetadata only
    Rapid conversion of carbon dioxide into titanium carbide by atmospheric microwave plasma
    (2024-01-01) Khotmungkhun K.; Kleebbua P.; Chotiyasilp A.; Waritanant T.; Atithep T.; Junpha J.; Subannajui K.; Khotmungkhun K.; Mahidol University
    The accelerating climate warming requires fast methods to reduce atmospheric carbon dioxide levels. Here, we converted carbon dioxide into titanium carbide using four magnetrons which were sequentially operated to emit microwave on titanium swarf. Carbon dioxide molecules dissociated in the plasma to react with ionized titanium atoms to form a stable titanium carbide product, using a microwave frequency is 2.3 gigahertz and 800 watts electrical power for each magnetron. Results show a reduction of carbon dioxide concentration from 2000 to 385 ppm within 30 s. Titanium carbide could be further functionalized as a three-dimensional printed gas sensor.
  • No Thumbnail Available
    ItemMetadata only
    Supramolecular self-assembled polymeric nanospheres based on hydrazino naphthalimide functionalised pillar[5]arene for long chain aldehyde detection
    (2023-01-01) Sanguansap Y.; Ruangpornvisuti V.; Atithep T.; Bunchuay T.; Tomapatanaget B.; Mahidol University
    A novel fluorescent sensor HNP5A is constructed consisting of bis-hydrazine naphthalimide decorated pillar[5]arene. Interestingly, this sensor possessed the potential for sensitive and selective detection of long-chain aldehydes, particularly nonanal (C9), and subsequently formed supramolecular pseudorotaxane polymeric nanoparticles inducing a strong fluorescence enhancement. In addition, this as-produced HNP5A⊂C9 exhibited an unexpected reduction of Ag+ to produce AgNPs in an aqueous system and the resulting AgNPs-HNP5A⊂C9 demonstrated a significant fluorescence enhancement under metal-enhanced fluorescence (MEF) behaviour.