Browsing by Author "Christiane Auray-Blais"

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    Analysis of trace metals in single droplet of urine by laser ablation inductively coupled plasma mass spectrometry
    (2011-10-01) Usarat Kumtabtim; Atitaya Siripinyanond; Christiane Auray-Blais; Aimé Ntwari; J. Sabine Becker; Forschungszentrum Jülich (FZJ); Mahidol University; Centre Hospitalier Universitaire de Sherbrooke
    Inductively coupled plasma mass spectrometry (ICP-MS) is now widely accepted as a universal and sensitive analytical technique in different research fields. In this project, we applied the technology to the analysis of complex matrix composition by using urine samples from Fabry disease patients and controls. The aims of the project were: (1) to develop a new and rapid analytical procedure for the determination of trace metal concentrations in single droplets of urine using laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS); and (2) to establish preliminary results for trace metal concentrations in Fabry disease patient urine samples and controls. The processing of samples required only drying a homogeneous urine sample. Different supports (or sample substrates) were used: Teflon sheets, Whatman 903 filter paper, Urine Kid paper and glass slides. In order to establish the merits of the analytical method developed, matrix-matched synthetic laboratory standards spiked with analytes of interest were prepared at low concentrations (μg L -1 level). The precision and accuracy of the analytical method were < 20% (LA-ICP-MS) for trace metals in 1 μL urine laboratory standards (at analyte concentrations of 300 ng mL -1 ). The limit of detection varied from 0.003 to 0.58 μg g -1 for lithium, boron, titanium, vanadium, chromium, manganese, cobalt, copper, zinc, arsenic, rubidium, str ontium, molybdenum, silver, cadmium, barium, lead, and uranium. LA-ICP-MS allowed the quantification and comparison of different trace metals in urine samples from a Fabry disease patient and from a reference control individual. This method may be applicable to forensic science, particularly when only a small amount of dried urine sample is available for investigation. © 2011 Elsevier B.V.
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    Mass spectrometry analysis of metals, other elements and lipids in urine samples of Fabry disease patients
    (2011-10-01) Christiane Auray-Blais; René Gagnon; Usarat Kumtabtim; Aimé Ntwari; J. Sabine Becker; Centre Hospitalier Universitaire de Sherbrooke; Forschungszentrum Jülich (FZJ); Mahidol University
    Fabry disease is an X-linked lysosomal storage disorder caused by deficiency of alpha-galactosidase A leading to accumulation of globotriaosylceramide in tissues and biological fluids of affected patients. Mass spectrometry is a powerful tool to quantify components of interest in biological fluids. Our study had four objectives: (1) to devise an ICP-MS methodology for quantitative determination of metal and other element concentrations in urine specimens of Fabry patients; (2) to analyze urinary Gb 3 /creatinine and lyso-Gb 3 /creatinine in these patients; (3) to evaluate correlations between urinary lipid concentrations versus metals and other elements in Fabry patients and healthy controls; (4) to evaluate which metals and other elements discriminate groups of patients and controls according to gender and treatment. We found that the excretion of barium was elevated in Fabry females and calcium and strontium levels were lower in Fabry males compared to controls. Preliminary results for treated and untreated Fabry disease patients indicate that ERT seems to have little effect on urine elements analyzed. Statistically significant correlations were established between urinary lyso-Gb 3 /creatinine, Gb 3 /creatinine and levels of magnesium, copper, mercury, nickel, lead, barium and calcium, whereas no significant correlations were found for the other 15 elements examined. Our results indicate that further studies are warranted in larger cohorts of Fabry disease patients for the investigation of possible roles of metals and other elements. © 2011 Elsevier B.V.