Browsing by Author "Dolurdee Boonsua"

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    Development of an amperometric immunosensor for the determination of methamphetamine in urine
    (2002-12-01) Kavinyanee Luangaram; Dolurdee Boonsua; Sarisak Soontornchai; Chamras Promptmas; Mahidol University; Sukhothai Thammatirat Open University
    An amperometric immunosensor in the competitive format was developed for the detection of methamphetamine in urine. The electrodes consisted of carbon paste and Ag/AgCl screen printed on heat sealing film, respectively, and of monoclonal lanti-methamphetamine antibody as the biorecognition element. Optimum amounts of methamphetamine-N-bovine serum albumin conjugate, monoclonal antibody and alkaline phosphatase-goat anti-mouse immunoglobulin G were 20, 10 ng and 1:10,000 dilution in 10 μl each, respectively. Methamphetamine was detected by the conversion of p-aminophenyl phosphate to electroactive p-aminophenol in the range of 200 ng/ml (lower detection limit) to 1,500 ng/ml methamphetamine in a nearly linear dose response curve. Within amphetamine concentrations of 0-1,500 ng/ml cross-reaction with methamphethamine was not observed. Working with urine samples spiked with methamphetamine, the accuracy and precision of the assay were 91.5-104.4% and 15.8-24.4%, respectively. This is a proof of concept in the clinical perspective for an amperometric immunosensor whose electrodes are amenable to future mass production.