Browsing by Author "Masatha Thongpan"

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    Analysis of Foxp3, CD25, and CD127 expressed on regulatory T cells in Thai subjects
    (2009-06-01) Masatha Thongpan; Chagriya Kitiyakara; Yada Louischaroen; Prasit Keesukphan; Sansanee C. Chaiyaroj; Mahidol University
    Foxp3+ natural regulatory T cells (nTreg) play a distinct role in maintaining self tolerance at the periphery. CD25hi and CD127 lo were proposed for the identification and purification of nTreg but they have not been confirmed in non-Caucasian populations. This study examined the sensitivity and purity of Foxp3 nTreg identified by CD25 and CD127 in the peripheral blood of Thai subjects (13 males, 15 females with age range of 20-42 years old). The proportions of nTreg/CD4+ as identified by the different markers were as follows: Foxp3+, 18.3 ± 6.4%; CD25hi, 6.4 ± 3.2%; and CD127lo, 54.3 ± 14.2%. Sensitivity tests showed the following results: CD25hi, 23.1%; CD127lo, 40.6%; CD25hiCD127lo, 7.4%. Purity tests concluded as follows: CD25hi, 63.6%; CD25int, 24.9%; CD25lo, 8.7%, CD127lo, 26.5%; CD127hi, 14.9%, and CD25hiCD127lo, 52.0%. In conclusion, the proportions of nTreg in Thai subjects are similar to Caucasian populations. CD25 hi is superior to CD127lo for separating Foxp3+ nTreg. Combining CD25hi and CD127lo does not improve the nTreg purity. Abbreviations: CD25hi, CD25 highly expressed cells; CD25int, CD25 intermediately expressed cells; CD25lo, CD25 low expressed cells; CD127hi, CD127 high expressed cells; CD127 lo, CD127 low expressed cells.
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    Natural Regulatory T cells (nTreg) in Minimal Change Disease and IgM Nephropathy
    (2012) Yada Louischaroen; Masatha Thongpan; Sansanee C. Chaiyaroj; Prasit Keesukphan; Panas Chalermsanyakorn; Vasant Sumethkul; Chagriya Kitiyakara; ญาดา หลุยเจริญ; มษฐา ทองปาน; ศันสนีย์ ไชยโรจน์; ประสิทธิ์ กี่สุขพันธ์; พนัส เฉลิมแสนยากร; วสันต์ สุเมธกุล; ชาครีย์ กิติยากร; Mahidol University. Faculty of Medicine Ramathibodi Hospital. Department of Medicine; Mahidol University. Faculty of Science; Mahidol University. Faculty of Medicine Ramathibodi Hospital. Department of Family Medicine; Mahidol University. Faculty of Medicine Ramathibodi Hospital. Department of Pathology
    Background: IgM nephropathy is a variant of minimal change disease (MCD) and is the commonest cause of nephrotic syndrome among Thai adults. Although patients with IgM nephropathy generally respond well to treatment with corticosteroids, patients often have a remitting and relapsing course. The pathogenesis is not well understood. It has been proposed that MCD or IgM nephropathy reflects a disorder of T-lymphocytes. Recently, abnormalities in natural regulatory T cells (nTreg) have been implicated in a number of autoimmune diseases. Objective: This is a pilot study to examine the number of Foxp3+CD4+ T cells in patients with MCD or IgM nephropathy. Methods: Eleven patients (2 patients with MDC and 9 patients with IgM nephropathy) and 25 normal control subjects were studied. T cell subpopulations were measured by flow cytometry. Results: Overall, nTreg were identified by the presence of Foxp3+CD4+ T cells. There were no significant differences in number of CD3+, CD4+ T cells population and Foxp3+CD4+ T cells in MCD/IgM patients compared with normal subjects. In a subgroup analysis, there was a significant increase in Foxp3+CD4+ numbers in untreated MCD/IgM patients with active disease compared to controls. Conclusion: These results suggesting correlation between activation of immune system in pathogenesis. Natural regulatory T cell as detected with Foxp3+CD4+ was increased in active MCD/IgM nephropathy patients.
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    Regulatory T cells in lupus glomerulonephritis
    (Mahidol University. Mahidol University Library and Knowledge Center, 2023) Masatha Thongpan; Sansanee Chaiyaroj; Chagriya Kitiyakara; Voravich Luangwedchakarn