Browsing by Author "Muhammad Nawaz"

Filter results by typing the first few letters
Now showing 1 - 3 of 3
  • No Thumbnail Available
    PublicationMetadata only
    Curcuma longa rhizome extract and Curcumin reduce the adhesion of Acanthamoeba triangularis trophozoites and cysts in polystyrene plastic surface and contact lens
    (2020-12-01) Watcharapong Mitsuwan; Suthinee Sangkanu; Chonticha Romyasamit; Chalermpon Kaewjai; Tajudeen O. Jimoh; Maria de Lourdes Pereira; Abolghasem Siyadatpanah; Sunil Kayesth; Muhammad Nawaz; Mohammed Rahmatullah; Mark S. Butler; Polrat Wilairatana; Christophe Wiart; Veeranoot Nissapatorn; Islamic University in Uganda; Imam Abdulrahman Bin Faisal university; The University of Nottingham Malaysia Campus; The University of Queensland; University of Delhi; Chulalongkorn University; Walailak University; Rangsit University; Birjand University of Medical Sciences; Centro de Investigação em Materiais Cerâmicos e Compósitos; Mahidol University; University of Development Alternative
    © 2020 The Authors Curcuma longa and Curcumin have been documented to have a wide spectrum of pharmacological effects, including anti-Acanthamoeba activity. Hence, this study sought to explore the anti-adhesion activity of C. longa extract and Curcumin against Acanthamoeba triangularis trophozoites and cysts in plastic and contact lenses. Our results showed that C. longa extract and Curcumin significantly inhibited the adhesion of A. triangularis trophozoites and cysts to the plastic surface, as investigated by the crystal violet assay (P < 0.05). Also, an 80–90% decrease in adhesion of trophozoites and cysts to the plastic surface was detected following the treatment with C. longa extract and Curcumin at 1/2 × MIC, compared to the control. In the contact lens model, approximately 1 log cells/mL of the trophozoites and cysts was reduced when the cells were treated with Curcumin, when compared to the control. Pre-treatment of the plastic surface with Curcumin at 1/2-MIC reduced 60% and 90% of the adhesion of trophozoites and cysts, respectively. The reduction in 1 Log cells/mL of the adhesion of A. triangularis trophozoites was observed when lenses were pre-treated with both the extract and Curcumin. Base on the results obtained from this study, A. triangularis trophozoites treated with C. longa extract and Curcumin have lost strong acanthopodia, thorn-like projection pseudopodia observed by scanning electron microscope. This study also revealed the therapeutic potentials of C. longa extract and Curcumin, as such, have promising anti-adhesive potential that can be used in the management/prevention of A. triangularis adhesion to contact lenses.
  • No Thumbnail Available
    PublicationMetadata only
    Nanotechnology applications of flavonoids for viral diseases
    (2021-11-01) Khoshnur Jannat; Alok K. Paul; Tohmina A. Bondhon; Anamul Hasan; Muhammad Nawaz; Rownak Jahan; Tooba Mahboob; Veeranoot Nissapatorn; Polrat Wilairatana; Maria de Lourdes Pereira; Mohammed Rahmatullah; Faculty of Tropical Medicine, Mahidol University; Imam Abdulrahman Bin Faisal university; Walailak University; University of Tasmania; CICECO – Instituto de Materiais de Aveiro; University of Development Alternative
    Recent years have witnessed the emergence of several viral diseases, including various zoonotic diseases such as the current pandemic caused by the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2). Other viruses, which possess pandemic-causing potential include avian flu, Ebola, dengue, Zika, and Nipah virus, as well as the re-emergence of SARS (Severe Acute Respiratory Syndrome) and MERS (Middle East Respiratory Syndrome) coronaviruses. Notably, effective drugs or vaccines against these viruses are still to be discovered. All the newly approved vaccines against the SARS-CoV-2-induced disease COVID-19 possess real-time possibility of becoming obsolete because of the development of ‘variants of concern’. Flavonoids are being increasingly recognized as prophylactic and therapeutic agents against emerging and old viral diseases. Around 10,000 natural flavonoid compounds have been identified, being phytochemicals, all plant-based. Flavonoids have been reported to have lesser side effects than conventional anti-viral agents and are effective against more viral diseases than currently used anti-virals. Despite their abundance in plants, which are a part of human diet, flavonoids have the problem of low bioavailability. Various attempts are in progress to increase the bioavailability of flavonoids, one of the promising fields being nanotechnology. This review is a narrative of some anti-viral dietary flavonoids, their bioavailability, and various means with an emphasis on the nanotechnology system(s) being experimented with to deliver anti-viral flavonoids, whose systems show potential in the efficient delivery of flavonoids, resulting in increased bioavailability.
  • No Thumbnail Available
    PublicationMetadata only
    Potential anti-Acanthamoeba and anti-adhesion activities of Annona muricata and Combretum trifoliatum extracts and their synergistic effects in combination with chlorhexidine against Acanthamoeba triangularis trophozoites and cysts
    (2021-05-01) Watcharapong Mitsuwan; Chea Sin; Samell Keo; Suthinee Sangkanu; Maria de Lourdes Pereira; Tajudeen O. Jimoh; Cristina C. Salibay; Muhammad Nawaz; Roghayeh Norouzi; Abolghasem Siyadatpanah; Christophe Wiart; Polrat Wilairatana; Polydor Ngoy Mutombo; Veeranoot Nissapatorn; Faculty of Tropical Medicine, Mahidol University; University of Puthisastra; Islamic University in Uganda; Imam Abdulrahman Bin Faisal university; The University of Nottingham Malaysia Campus; De La Salle University; Chulalongkorn University; Walailak University; Birjand University of Medical Sciences; CICECO – Instituto de Materiais de Aveiro; University of Tabriz; Academic Center for Education and Training (ACET); Neglected Tropical Diseases
    Plants with medicinal properties have been used in the treatment of several infectious diseases, including Acanthamoeba infections. The medicinal properties of Cambodian plant extracts; Annona muricata and Combretum trifoliatum were investigated against Acanthamoeba triangularis. A total of 39 plant extracts were evaluated and, as a result, 22 extracts showed positive anti-Acanthamoeba activity. Of the 22 extracts, 9 and 4 extracts showed anti-Acanthamoeba activity against trophozoites and cysts of A. triangularis, respectively. The minimum inhibitory concentration of A. muricata and C. trifoliatum extracts against trophozoites and cysts was 500 and 1,000 μg/mL, respectively. The combination of A. muricata at 1/4×MIC with chlorhexidine at 1/8×MIC demonstrated a synergistic effect against trophozoites, but partial synergy against cysts. A 40% reduction in trophozoites and 60% of cysts adhered to the plastic surface treated with both extracts at 1/2×MIC were noted comparing to the control (P < 0.05). Furthermore, a reduction of 80% and 90% of trophozoites adhered to the surface was observed after pre-treatment with A. muricata and C. trifoliatum extracts, respectively. A 90% of cysts adhered to the surface was decreased with pre-treatment of A. muricata at 1/2×MIC (P < 0.05). A 75% of trophozoites and cysts from Acanthamoeba adhered to the surface were removed after treatment with both extracts at 4×MIC (P < 0.05). In the model of contact lens, 1 log cells/mL of trophozoites and cysts was significantly decreased post-treatment with both extracts compared to the control. Trophozoites showed strong loss of acanthopodia and thorn-like projection pseudopodia, while cysts demonstrated retraction and folded appearance treated with both extracts when observed by SEM, which suggests the potential benefits of the medicinal plants A. muricata and C. trifoliatum as an option treatment against Acanthamoeba infections.