Browsing by Author "Noppawan Tungbuppha"

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    Hb tak and Hb Q-Thailand in thai patients are S-window hemoglobin variants revealed by high performance liquid chromatography
    (2010-04-01) Sumalee Jindadamrongwech; Noppawan Tungbuppha; Suporn Chuncharunee; Punnee Butthep; Faculty of Medicine, Ramathibodi Hospital, Mahidol University; Mahidol University
    The S-window hemoglobin (Hb) variants revealed by high performance liquid chromatography (HPLC) were studied in 12 Thai individuals. The variants were identified, using DNA sequencing and multiplex amplification refractory mutation system-polymerase chain reaction (ARMS-PCR), to be six cases of Hb Tak [β147 (AC)], and six cases of Hb Q-Thailand [α74(EF3)Asp→His], respectively. By using the Capillarys 2-capillary zone electrophoresis (CE), Hb Tak and Hb Q-Thailand co-migrated with Hb F in zone 7. This might pose a problem as the high Hb F conditions suggest a differential diagnosis. The S-window Hb variants are mostly Hb Tak and Hb Q-Thailand in the Thai population rather than Hb S [β6(A3)Glu→Val]. The definite identification of Hb variants detected by HPLC or capillary electrophoresis (CE) requires DNA analysis. © Informa UK, Ltd.