Browsing by Author "Pham V.K."

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    Analysis of the Mechanical and Biofilm-Inhibitory Antimicrobial Properties of a Dental Tissue Conditioner Incorporating Ocimum Gratissimum Essential Oil: An In Vitro Study
    (2025-01-01) Doan T.M.; Vu C.T.B.; Truong P.T.L.; Pham V.K.; Chotprasert N.; Doan T.M.; Mahidol University
    Objectives: This study aimed to investigate the tensile bond strength (TBS) and biofilm-inhibitory antimicrobial properties of tissue conditioners combined with Ocimum gratissimum essential oil (EO) at varying concentrations. Materials and Methods: The original tissue conditioner was used as the control, while the experimental groups consisted of tissue conditioners incorporating O. gratissimum EO at concentrations of 1% and 2% (v/v) in the liquid component. The TBS between the tissue conditioner and denture base acrylic resin was measured using a universal testing machine. To evaluate biofilm-inhibitory antimicrobial properties, cylindrical specimens infused with EO were prepared and incubated with Candida albicans and Streptococcus mutans. The crystal violet assay was utilized to quantify microbial biofilm formation. Results: The incorporation of O. gratissimum EO into tissue conditioners significantly increased the TBS on day 1 (p < 0.05) but showed no effect by day 7 post-polymerization (p > 0.05). Additionally, tissue conditioners containing 1% EO exhibited biofilm-inhibitory antimicrobial properties on day 1 (p < 0.05), whereas those with 2% EO demonstrated biofilm-inhibitory antimicrobial activity on both days 1 and 3 postinoculation (p < 0.05). By day 5 and 7, EO-infused tissue conditioners no longer exhibited biofilm-inhibitory antimicrobial properties (p > 0.05). Conclusion: Tissue conditioners infused with O. gratissimum EO effectively reduced the formation of biofilms by C. albicans and S. mutans in a dose–dependent manner on days 1 and 3. As tissue conditioners are typically replaced every 3–7 days, O. gratissimum EO can be incorporated as an additive to lower the formation of biofilms by C. albicans and S. mutans without compromising the TBS of the tissue conditioner to denture base acrylic resin.
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    Comparative analysis of the fit quality of monolithic zirconia veneers produced through traditional and digital workflows using silicone replica technique: an in vitro study
    (2024-12-01) Doan T.M.; Nguyen T.N.; Pham V.K.; Chotprasert N.; Vu C.T.B.; Doan T.M.; Mahidol University
    Background: The success of a restoration largely depends on the quality of its fit. This study aimed to investigate the fit quality of monolithic zirconia veneers (MZVs) produced through traditional and digital workflows. Methods: A typodont maxillary right central incisor was prepared. The maxillary arch with the prepared tooth was scanned with Trios 3 Pod intra-oral scanner (IOS), which served as a pattern to create thirty 3D resin models through printing. Additionally, thirty conventional impressions of the maxillary with the prepared tooth were taken using polyvinyl siloxane (PVS) impression material. These impressions were cast using dental gypsum products to create thirty stone dies, which were then scanned externally. Sixty MZVs were milled from multi-layered zirconia disks. The marginal and internal gaps of restorations were assessed using the silicone replica technique. Results: The highest marginal accuracy for both the conventional and digital impression groups was observed in the cervical area, with values of 74.6 μm and 61.9 μm, respectively. The smallest internal gaps for both groups were also recorded in the cervical area, at 109.9 μm for the conventional group and 109.7 μm for the digital group. The digital group exhibited better marginal fit, particularly in the incisal and mesial areas (79.3 μm and 75.7 μm, respectively), compared to the conventional group (88.1 μm and 90.8 μm). No statistically significant differences in internal fit were observed. Conclusion: MZVs fabricated using the digital workflow exhibited superior marginal fit compared to those fabricated using the conventional workflow, though both techniques yielded clinically acceptable results.