Browsing by Author "Rutjawate Taharnklaew"

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    Characterization of flagellotropic, chi-like salmonella phages isolated from thai poultry farms
    (2019-06-01) Preeda Phothaworn; Matthew Dunne; Rattaya Supokaivanich; Catherine Ong; Jiali Lim; Rutjawate Taharnklaew; Mongkol Vesaratchavest; Rabuesak Khumthong; Onanong Pringsulaka; Pravech Ajawatanawong; Jochen Klumpp; Nathan Brown; Mohammed Imam; Martha R.J. Clokie; Edouard E. Galyov; Sunee Korbsrisate; University of Leicester; ETH Zürich; Mahidol University; Faculty of Medicine, Siriraj Hospital, Mahidol University; DSO National Laboratories; Srinakharinwirot University; Betagro Science Center Co. Ltd.
    © 2019 by the authors. Licensee MDPI, Basel, Switzerland. Despite a wealth of knowledge on Salmonella phages worldwide, little is known about poultry-associated Salmonella phages from Thailand. Here, we isolated 108 phages from Thai poultry farms that infect Salmonella enterica serovar Typhimurium. Phages STm101 and STm118 were identified as temperate Siphoviridae phages. Genome sequencing and analyses revealed these phages share approximately 96% nucleotide sequence similarity to phage SPN19, a member of the Chi-like virus genus. PCR amplification of the gene encoding capsid protein E of the Chi-like phage was positive for 50% of phage isolates, suggesting a predominance of this phage type among the sampled poultry farms. In addition to the flagella, two phages required the lipopolysaccharide to infect and lyse Salmonella. Furthermore, phylogenomic analysis demonstrated that phages STm101 and STm118 formed a monophyletic clade with phages isolated from Western countries, but not from closer isolated phages from Korea. However, further investigation and more phage isolates are required to investigate possible causes for this geographic distribution.