Browsing by Author "S. Chariyalertsak"

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    Aberrant cyclooxygenase isozyme expression in human intrahepatic cholangiocarcinoma
    (2001-01-19) S. Chariyalertsak; V. Sirikulchayanonta; D. Mayer; A. Kopp-Schneider; G. Fürstenberger; F. Marks; K. Müller-Decker; National Cancer Institute Thailand; Mahidol University; German Cancer Research Center
    Methods - Cellular localisation of the cyclooxygenase (COX) isozymes COX-1 and COX-2 was analysed in 24 cholangiocarcinomas, including 17 matched tissues originating from non-tumorous liver tissue adjacent to tumours and seven biopsies of normal human liver, by immunohistochemistry using isozyme selective antibodies. Results - In normal liver, constitutive expression of COX-2 protein was a characteristic feature of hepatocytes whereas no COX-2 immunosignal was detectable in normal bile duct epithelium, Kupffer, and endothelial cells. In cholangiocarcinoma cells, COX-2 protein was strongly expressed at high frequency. The intensity, percentage of positive cells, and pattern of COX-2 expression were found to be independent of the stage of tumour differentiation. In hepatocytes of matched nontumorous tissue, COX-2 expression was unaltered. In contrast, strong COX-1 expression was frequently localised to Kupffer cells, endothelial cells, and occasionally to hepatocytes, but not to bile duct epithelial cells. In approximately half of moderately and poorly differentiated but not well differentiated cholangiocarcinomas, weak to moderate COX-1 staining was found in tumour cells while COX-1 expression in Kupffer cells was much more pronounced. Conclusion - Aberrant COX-2 expression occurs during the early stage while COX-1 over expression seems to be related to later stages of cholangiocarcinogenesis.
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    Allelic loss on chromosome 5q34 is associated with poor prognosis in hepatocellular carcinoma
    (2008-10-01) P. Saelee; S. Wongkham; V. Bhudhisawasdi; B. Sripa; S. Chariyalertsak; S. Petmitr; Mahidol University; Khon Kaen University; National Cancer Institute Thailand
    Purpose: To identify and characterize novel genetic alterations in hepatocellular carcinoma (HCC). Methods: DNA was extracted from 29 HCC and corresponding normal tissues and amplified with 59 different 10-base arbitrary primers. A 550 bp DNA fragment amplified using primer Q-9 and which was present in 19 of 29 cases (66%) was cloned, sequenced, and compared with known nucleotide sequences deposited in Genome database, and quantified by real-time PCR. Results: DNA alterations were found on chromosomes 5q34, 6p25.2 and 8q12.1 in 11 of 29 cases (38%), 7 of 29 cases (24%), and 12 of 29 cases (41%), respectively. Multivariate analysis showed that the allelic loss on chromosome 5q34 was an independent prognostic factor for poor survival of HCC patients, with the median survival time of 19 weeks for allelic loss versus 109 weeks for no allelic loss (P = 0.001). Conclusions: This study indicates that allelic loss on chromosome 5q34 may be involved in the development of HCC and could be used as a prognostic indicator in HCC patients. © 2008 Springer-Verlag.
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    Dietary Pattern and Metabolic Syndrome in Thai Adults
    (2015-01-01) W. Aekplakorn; W. Satheannoppakao; P. Putwatana; S. Taneepanichskul; P. Kessomboon; V. Chongsuvivatwong; S. Chariyalertsak; Mahidol University; National Health Examination Survey Office; Chulalongkorn University; Khon Kaen University; Prince of Songkla University; Chiang Mai University
    © 2015 W. Aekplakorn et al. Objectives. To determine the dietary patterns of middle-aged Thais and their association with metabolic syndrome (MetS). Methods. The Thai National Health Examination Survey IV data of 5,872 participants aged ≥30-59 years were used. Dietary patterns were obtained by factor analysis and their associations with Mets were examined using multiple logistic regression. Results. Three major dietary patterns were identified. The first, meat pattern, was characterized by a high intake of red meat, processed meat, and fried food. The second, healthy pattern, equated to a high intake of beans, vegetables, wheat, and dairy products. The third, high carbohydrate pattern, had a high intake of glutinous rice, fermented fish, chili paste, and bamboo shoots. Respondents with a healthy pattern were more likely to be female, higher educated, and urban residents. The carbohydrate pattern was more common in the northeast and rural areas. Compared with the lowest quartile, the highest quartile of carbohydrate pattern was associated with MetS (adjusted odds ratio: 1.82; 95% CI 1.31, 2.55 in men and 1.60; 95% CI 1.24, 2.08 in women), particularly among those with a low level of leisure time physical activity (LTPA). Conclusion. The carbohydrate pattern with low level of LTPA increased the odds of MetS.
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    Mortality after hip fractures in Thailand
    (2001-01-01) S. Chariyalertsak; P. Suriyawongpisal; A. Thakkinstain; Chiang Mai University; Mahidol University
    Hip fractures have long been considered a major threat to the survival of elderly people. Most of the studies on survival following hip fractures have been reported from developed countries where orthopedic care is well distributed. This report describes the survival experience of 330 elderly hip fracture patients who were seen at hospitals in Chiang Mai, Thailand. The mortality rate during hospitalization was 2.1%. The 3-, 6-, and 12-month survival rates after hip fractures were 91%, 88% and 83%, respectively. The significant predictors of mortality were male sex, age over 80 years, presence of chronic illnesses, poor pre-fracture walking ability, and nonoperative treatment.
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    Novel DNA amplification on chromosomes 2p25.3 and 7q11.23 in cholangiocarcinoma identified by arbitrarily primed polymerase chain reaction
    (2005-12-01) S. Chariyalertsak; T. Khuhaprema; V. Bhudisawasdi; B. Sripa; S. Wongkham; S. Petmitr; Mahidol University; National Cancer Institute Thailand; Khon Kaen University
    Purpose: To detect and characterize amplified DNA sequences in cholangiocarcinoma (CCA). Patients and methods: We extracted DNA from tumor and corresponding normal tissues of 30 patients with CCA and amplified with 30 random ten-mer arbitrary primers by the arbitrarily primed polymerase chain reaction (AP-PCR) technique. Results: Our results showed gains of genomic sequences at high frequency. Using the AX-11 arbitrary primer, we determined an amplified DNA fragment occurred frequently in the tumors analyzed. The DNA fragment was isolated and identified as two sequences mapped to chromosomes 2p25.3 and 7q11.23. Specific primers were designed employing these sequences and used for detecting amplification by real-time quantitative PCR. The amplification of the DNA sequences on chromosomes 2p25.3 and 7q11.23 was detected in 10 (33%) and 6 (20%) cases, respectively. Thirteen (43%) cases showed amplification on both or one of the chromosomes. In addition, amplification of the DNA on chromosome 2p25.3 was predominantly observed in poorly differentiated tumors. Conclusions: Our findings suggest that the novel amplified DNA on chromosomal regions at 2p25.3 and 7q11.23 might be involved in the development and progression of CCA. © Springer-Verlag 2005.