Browsing by Author "S. Ploybutr"

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    Comparison between colorimetric and column chromatographic methods for the determination of glycosylated hemoglobin
    (1984-12-01) A. Vichayanrat; S. Ploybutr; W. Nitiyanant; S. Vannasaeng; Mahidol University
    We reported a study on glycosylated hemoglobin (GHb) determined by colorimetry (TBA method) and column chromatography in 82 diabetics and normal subjects. There was a good correlation between the GHb values measured by both methods (r = 0.879). An equation was derived from the curve and used to convert GHb by colorimetry in μmol/l HMF to percentage of total hemoglobin. In another group of 36 normal subject and 190 diabetics, GHb concentrations were determined by the colorimetric method and expressed as percentage of total hemoglobin. The mean GHb values of these 36 normal Thai subjects, 20 untreated diabetics, 70 diabetics on insulin therapy and 100 diabetics on oral hypoglycemic agents were 6.64, 14.01, 13.59 and 11.70 per cent respectively. All diabetics had a significantly higher mean GHb value compared to normal subjects. The patients on oral hypoglycemic agents had a significantly lower GHb concentration when compared to untreated and insulin requiring diabetics. From our experience, GHb determination by this colorimetric method expressed as percentage of total hemoglobin is accurate, convenient to standardize and inexpensive, and therefore it is a suitable procedure for GHb measurement.
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    Effects of alpha-glucosidase inhibitor (acarbose) combined with sulfonylurea or sulfonylurea and metformin in treatment of non-insulin-dependent diabetes mellitus
    (1995-12-01) S. Vannasaeng; S. Ploybutr; W. Nitiyanant; T. Peerapatdit; A. Vichayanrat; Mahidol University
    The effects of alpha-glucosidase inhibitor (acarbose) were studied in 36 patients with non-insulin-dependent diabetes mellitus (NIDDM), aged 34-67 years with a mean duration of diabetes of 8.8 ± 0.9 years. They were poorly controlled with diet plus sulfonylurea alone or plus sulfonylurea combined with metformin drugs. Acarbose, 100 mg three times daily, was additionally given to these patients for six months. Results showed small but significant decreases (P < 0.001) in postprandial blood glucose level. Glycosylated hemoglobin level was lowered significantly (P < 0.001) and was normalised (level of < 8%) in 17 per cent of the patients. Fasting serum triglycerides level decreased significantly (P < 0.01), whereas, no significant changes in serum total cholesterol and HDL cholesterol levels were seen. Body weight also decreased significantly (P < 0.001) at the end of acarbose trial. Flatulence was the major side effect of acarbose found in 42 per cent of the patients but it was well-tolerated and may be transient and self-limited. We concluded that the addition of acarbose to the therapeutic regimens of diet therapy plus sulfonylurea or plus sulfonylurea combined with metformin drugs led to significant improvement of glycemic control. Acarbose may be a safe and valuable adjunct to diet and sulfonylurea and metformin treatments in obese, poorly-controlled patients with NIDDM.
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    Endogenous estradiol in elderly individuals: Cognitive and noncognitive associations
    (2002-01-01) V. Senanarong; S. Vannasaeng; N. Poungvarin; S. Ploybutr; S. Udompunthurak; P. Jamjumras; L. Fairbanks; J. L. Cummings; Faculty of Medicine, Siriraj Hospital, Mahidol University; Mahidol University; David Geffen School of Medicine at UCLA
    Objective: To investigate an association between endogenous estradiol (E2) levels and cognition and behavior in elderly individuals. Patients: We studied 135 community-based men and women aged 52 to 85 years in urban Bangkok, Thailand; 72 had dementia and 63 did not. Materials and Methods: Dementia was diagnosed using Diagnostic and Statistical Manual of Mental Disorders, Fourth Edition, criteria after appropriate investigations. Blood samples for assay were collected in the morning after 6 hours of fasting. Levels of E2were measured by radioimmunoassay (double antibody technique). The Thai version of the Mini-Mental State Examination was used to assess cognition; the Neuropsychiatric Inventory was used to assess neuropsychiatric symptoms; and the Functional Assessment Questionnaire was used to assess instrumental activities of daily living. Results: There was no correlation between age and level of E2in either men or women. Individuals with lower estrogen levels had more behavioral disturbances (men: r = -0.467, n = 45; P = .001; women: r= -0.384, n = 90; P<.001) and worse cognition (men: r = 0.316, n = 45; P = .03; women: r = 0.243, n = 90; P= .02) and function (men: r = -0.417, n = 45; P =.004; women: r = -0.437, n = 90; P<.001). The threshold level of endogenous E2in elderly individuals for the risk of developing dementia was less than 15 pg/mL (<55 pmol/L) in men and less than 1 pg/mL (<4 pmol/L) in women. Conclusion: Lower E2levels are correlated with poor cognitive, behavioral, and functional status in older individuals.
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    Glycosylated hemoglobin by colorimetry in normal subjects, diabetics, and nondiabetics
    (1984-12-13) A. Vichayanrat; S. Ploybutr; W. Nitiyanant; S. Vannaseang; S. Tandhanand; Mahidol University
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    Isolated growth hormone deficiency: A study in 14 Thai patients
    (1982-12-01) S. Vannasaeng; A. Vichayanrat; S. Ploybutr; M. Inthuprapa; S. Tandhanand; Mahidol University
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    Nocturnal 8mg dexamethasone suppression test: A practical and accurate test for identification of the cause of endogenous Cushing's syndrome
    (1996-02-14) S. Sriussadaporn; S. Ploybutr; T. Peerapatdit; Mahidol University
    The efficacy of a nocturnal 8mg dexamethasone suppression test (nocturnal DST) was compared with that of the standard high-dose dexamethasone suppression test (standard DST) in identifying the cause of endogenous Gushing's syndrome in 10 proven cases with Gushing's disease, 20 with adrenal tumours, and one with ectopic ACTH syndrome. The nocturnal test compared serum cortisol concentration at 8am before and after administration of a single dose of 8mg dexamethasone at 11pm. Suppression of serum cortisol level to < 50% of the baseline value indicated a diagnosis of Gushing's disease, while a lack of suppression below that limit indicated one of the other two causes of Gushing's syndrome: glucocorticoid-secreting adrenal tumour or ectopic ACTH syndrome. The nocturnal DST had a sensitivity of 90%, a specificity of 100%, an accuracy of 96.8%, a positive predictive value of 100%, and a negative predictive value of 95.5%. These values are comparable to the efficacy of the standard DST in distinguishing Gushing's disease from glucocorticoid-secreting adrenocortical tumour or ectopic ACTH syndrome. Furthermore, this rapid test does not require hospitalisation or urine collection like the standard DST. The nocturnal 8mg dexamethasone suppression test is practical, fairly reliable, and an effective alternative with which to identify the cause of endogenous Gushing's syndrome.
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    On the comparison of serum cortisol by direct radioimmunoassay and fluorometric method
    (1982-12-01) T. Kanluan; S. Ploybutr; A. Vichayanrat; Mahidol University