Browsing by Author "S. Soontornyatara"

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    Effect of different combinations of NAA and TDZ for shoot induction in vitro culture of Aglaonema simplex (Blume) Blume
    (2020-12-08) S. Soontornyatara; P. Klammorn; Mahidol University
    © 2020 International Society for Horticultural Science. All rights reserved. Wan Khanmak (Aglaonema simplex (Blume) Blume) is a kind of Thai herb. The ripe fruits are extensively used as longevity enhancer and anti-asthma medication, but the number of fruits plant-1was only 3-14. This research project aims to find the most appropriate tissue culture protocol for mass propagation of Wan Khanmak herb. The experimental design was completely randomized design (CRD). The axillary buds of Wan Khanmak were incubated on MS medium supplemented with 1-naphthaleneacetic acid (NAA) and thidiazuron (TDZ) at various doses; 0:0, 0.25:0.5, 0.25:1.0, 0.25:1.5, 0.25:2.0 and 0.25:2.5 mg L-1. The results showed that the most effective medium was MS medium supplemented with NAA and TDZ at 0.25:2.5 mg L-1ratio which produced the highest number of average shoots per culture (4.49 shoots) with an average shoots length 1.60 cm in 8 weeks of culture. This treatment gave the fastest time to produce the first shoot in 8.5 days.
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    Effect of gamma ray on morphological characteristic of Nelumbo nucifera (Roseum Plenum lotus)
    (2017-07-21) S. Soontornyatara; P. Singhavorachai; V. Puripunyavanich; P. Taywiya; Mahidol University; Thailand Institute of Nuclear Technology (TINT)
    © 2017, International Society for Horticultural Science. All rights reserved. Effect of gamma ray on morphological characteristics of Nelumbo nucifera (Roseum Plenum lotus or sacred lotus) was investigated. The lotus seeds were acutely irradiated with the doses of 0, 200, 400, 600, 800 and 1,000 Gy and the lotus rhizomes were acutely irradiated with the doses of 0, 10, 20, 30, 40 and 50 Gy. The irradiated seeds and rhizomes were planted in plastic pots for 30 days. The germination rates of gamma-ray exposed seeds and control were observed. The results showed that some of the irradiated seeds were able to germinate. After that, all seedlings failed to grow. The germination rates of irradiated rhizomes with the doses of 0, 10 and 20 Gy were 100, 55.56 and 22.22%, respectively. LD50was established at about 12 Gy. The morphological characteristics of lotus plants from irradiated rhizomes were different from the original lotus plants, such as undulate leaves, pink or yellow scattered spots on leaves and brown or reddish-brown young leaves. This experiment gave basic information which was very useful for lotus mutation breeding.
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    Effect of gamma rays on morphological character variation of Nelumbo nucifera ‘Pathum’
    (2017-07-21) S. Soontornyatara; P. Sornjai; V. Puripunyavanich; P. Taywiya; Mahidol University; Thailand Institute of Nuclear Technology (TINT)
    © 2017, International Society for Horticultural Science. All rights reserved. Nelumbo nucifera Gaertn., common name: lotus, is an aquatic plant which is widely grown in East and South-East Asian countries. Lotus flower has had only two colors, pink and white morphological characteristics for over 100 years. The major problem of this ornamental plant is its limited morphological characters for economic purposes. Thus, this project aims are to study the effect of acute gamma rays on morphological character and chromosome variation of N. nucifera cultivar ‘Pathum’. The lotus seeds were irradiated at doses of 0, 10, 20, 30, 40, 50, 60, 70, 80, 90, and 100 Krad. The exposed seeds were cultured in plastic pots for 60 days. The results showed that the lethal dose at 50% (LD50) was 28 Krad. The 50 Krad dose or higher were lethal for seed germination. The gamma doses of 10, 20, 30 and 40 Krad produced 14, 12, 14, and 5 various abnormal characters, respectively. The morphological characters of seedlings from radiated lotus seeds were twin leaves and different types of dispersed spot leaves. The chromosome number of lotus was also studied and the results showed no difference between irradiated and control plants. The optimal radiation dose of gamma irradiation was to be around 20-30 Krad. This experiment provides basic information for N. nucifera ‘Pathum’ mutation breeding.
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    Reflecting properties of ornamental plants for directing traffic in public park
    (2020-12-08) S. Soontornyatara; S. Phanchai; K. Khaobang; Mahidol University
    © 2020 International Society for Horticultural Science. All rights reserved. An effective way to reduce electricity consumption is planting of ornamental plants that have light-reflecting properties along the traffic path in public parks. This research project focused on 60 ornamental plant species that have light-reflecting properties. Plant samples were categorized into three groups; tree, shrub, and ground cover group. The experiment was arranged in a completely randomized design (CRD) with 10 replications. The experiment was carried out in a 1.45 m chamber that adjusted the condition to imitate the surrounding of a public park at night. Ten mature leaves per sample were collected and then placed at the center of the completely dark room. After that, the light bulb was turned on for 20 min. A light meter, model LI-250A was used as a tool for measuring the intensity level of the leaf reflected-light. The results revealed that the most light-reflective leaf of each plant group, the tree group was Pisonia grandis, with an average reflectance level of 5.66 lx. For the small shrub group, the highest average light intensity of 5.73 lx was obtained from Dieffenbachia seguine 'Tropic Snow? For the ground cover group, Pandanus baptistii 'Aureus?showed the highest average reflective of 6.16 lx. These three species are the most appropriate plant for directing traffic in public park.