Browsing by Author "Sasithorn Lorroengsil"

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    Genetic differences of E gene of A Thai strain of Japanese encephalitis virus that determine small plaque size phenotype but not neurovirulence in suckling mice
    (2008-05-01) Sasithorn Lorroengsil; Antony Chen; Matti Sällberg; Somsak Pantuwatana; Mahidol University; Ubon Rajathanee University; Karolinska Institutet; Burapha University
    Two small plaque variants of Japanese encephalitis virus (JEV), S4P9 and S9P10, were recovered from the wild type of JEV strain KE-093 using plaque purification in combination with the temperature-shift induction technique. Growth patterns of the S4P9 and S9P10 in BHK-21 cells as well as neurovirulence in suckling mice were similar to that of KE-093. An amino acid substitution, lysine for glutamic acid, was present in envelope protein at residue E-83 in the small plaque variants. This study shows that small plaque phenotype is not always associated with attenuation in vivo.