Browsing by Author "Tantarungsee N."

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    Genotypic Analysis of ABCA4 Coding Sequence in Thai Patients with Stargardt Disease
    (2024-01-01) Vatanashevanopakorn C.; Soi-ampornkul R.; Chaisidhivej N.; Sompohnmanas A.; Dhirachaikulpanich D.; Tantarungsee N.; Piampradad S.; Prakhunhungsit S.; Phasukkijwatana N.; Vatanashevanopakorn C.; Mahidol University
    Objective: To study the mutational spectrum of the ABCA4 gene in Thai patients with Stargardt disease. Materials and Methods: DNA sequencing of all 50 exons of the ABCA4 gene was performed in nine Thai patients with clinically diagnosed Stargardt disease. Results: Amino acid sequence variations in the ABCA4 gene were found in five patients. Six missense mutations, c.71G>A, c.635G>A, c.1268A>G, c.3626T>C, c.4283C>T, and c.5761G>A, previously associated with Stargardt disease, were identified in our cohort. The variant c.1268A>G was the most prevalent in our study. Conclusion: In this cohort, only 56% of Thai Stargardt patients had missense mutations in the ABCA4 gene. Mutations in the non-coding regions of the ABCA4 or mutations in other genes may be responsible for Stargardt phenotypes in the remaining patients. Our findings are the first to reveal the mutational spectrum of ABCA4 leading to Stargardt disease in the Thai population and demonstrate a potential for ABCA4 screening as well as the importance of genetic variability in Thai patients with clinically suspected Stargardt disease.
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    Long-Term Paracetamol Treatment Impairs Cognitive Function and Brain-Derived Neurotrophic Factor in Adult Rat Brain
    (2023-01-01) Lalert L.; Tantarungsee N.; Chotipinit T.; Ji-au W.; Srikiatkhachorn A.; Maneesri-le Grand S.; Mahidol University
    Paracetamol (acetaminophen, APAP) is known as a safe pain reliever; however, its negative effects on the central nervous system have gradually been reported. We examined alterations in learning and memory, and brain-derived neurotrophic factor (BDNF) expression in the frontal cortex and hippocampus at different durations of APAP treatment in rats. Novel object recognition (NOR) and Morris water maze (MWM) paradigms were used to assess learning and memory in rats fed with 200 mg/kg APAP at single-dose, 15-day or 30-day treatments. BDNF expression was evaluated through immunohistochemistry and Western blotting. The single-dose APAP treatment did not alter the NOR performance. However, deficits in the NOR and MWM capacities were detected in the rats with longer durations of APAP treatment. An analysis of BDNF expression revealed no significant change in BDNF expression in the single-dose APAP treatment, while rats given APAP for extended periods as treatment showed a significant decrement in this protein in the frontal cortex and hippocampus. Short-term APAP treatment has no effect on learning and memory, or BDNF expression; however, long-term APAP exposure causes cognitive impairment. The diminishment of the BDNF level in the frontal cortex and hippocampus due to the long period of treatment with APAP may at least in part be involved in altered learning and memory in rats.
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    MicroRNA profiles of four induced pluripotent stem cell lines derived from distinct tissues
    (2025-12-01) Trakarnsanga K.; Vorasan N.; Tantarungsee N.; Wattanapanitch M.; Vatanashevanopakorn C.; Suktitipat B.; Metheetrairut C.; Trakarnsanga K.; Mahidol University
    Objective: Induced pluripotent stem cells (iPSCs) are generated from a vast number of adult cell types. While they all acquired embryonic stem cell (ESC)-like properties during reprogramming, differences in certain characteristics, including differentiation potential, remained. These differences are hypothesized to be due to epigenetic memory or individual genetic background. Results: This study compared microRNA (miRNA) profiles, which is one aspect of epigenetic regulation, of four iPSC lines derived from dermal fibroblasts, hair follicle keratinocytes, peripheral blood T cells, and exfoliated renal epithelial cells by nCounter® miRNA expression panels. 110 miRNAs were expressed in all iPSC lines, which accounted for 67.5–75.9% of expressed miRNAs in each line, while there were only 3, 4, 9, and 28 uniquely expressed miRNAs in each line. MiRNA profiles of the four iPSC lines clustered together, but away from those of mature cells of origin or ESCs retrieved from the Gene Expression Omnibus database as analyzed by principal component analysis. These results suggested that the miRNA profiles of the four iPSC lines are mostly similar to each other, though with some specific unique gene expression, and do not appear to indicate any obvious differences between the iPSC lines derived from different adult cell types in terms of miRNA expression.
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    N-trans-feruloyltyramine Protects Human Neuroblastoma SK-N-SH Cell Line Against H2O2-Induced Cytotoxicity
    (2022-08-01) Soi-ampornkul R.; Myint E.E.P.; Thangnipon W.; Tantarungsee N.; Mitrpant C.; Tuchinda P.; Nobsathian S.; Vatanashevanopakorn C.; Mahidol University
    Excessive accumulation of reactive oxygen species (ROS) has been regarded as a major contributor of pathogenesis in neurodegenerative diseases. N-trans-feruloyltyramine (NTF), an alkaloid isolated from several plants, has demonstrated an ability to be a potent antioxidant. In this study, the antioxidative and anti-apoptotic properties of NTF extracted from the stems of Polyalthia suberosa were investigated in the human neuroblastoma cell line SK-N-SH. NTF at concentrations ranging from 10 µM to 500 µM were not toxic to cells and reduced intracellular ROS levels significantly. Furthermore, pre-treatment of NTF significantly decreased H2O2-induced ROS generation and attenuated H2O2-mediated cytotoxicity. An increase in the expression of Bax and activated caspase-3 and reduction of Bcl-2 mediated by H2O2 was reversed by pre-treating the cells with 100 µM NTF. Likewise, NTF suppressed the increase of caspase-3 activity induced by H2O2. In conclusion, the findings reveal that NTF improves H2O2-induced intracellular ROS generation and decreases apoptosis. These protective effects of NTF could be useful for oxidative stress-related neurodegenerative conditions.