Browsing by Author "Viet Khong Nguyen"

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    Acute febrile myalgia in vietnam due to trichinellosis following the consumption of raw pork
    (2009-10-01) Walter R.J. Taylor; Giang Van Tran; Thai Quoc Nguyen; Duong Van Dang; Viet Khong Nguyen; Cap Trung Nguyen; Lam Tien Nguyen; Chinh Quoc Luong; Teresa Scott; Dang Thi Cam Thach; Tran Thi Ha Ninh; Thang Danh Nguyen; Khoung Thi Pham; Annette Fox; Peter Horby; Heiman Wertheim; Doan Hanh Nhan; Hong Ha Nguyen; Lien Minh Thi Trinh; Trung Vu Nguyen; Kinh Van Nguyen; Due Hien Nguyen; University of Oxford; National Institute for Infectious and Tropical Diseases; Bach Mai Hospital; National Institute of Veterinary Research; National Institute for Malariology; Nuffield Department of Clinical Medicine; King's College London; Mahidol University
    Trichinellosis outbreaks occur occasionally in Vietnam following the consumption of undercooked pork. Diagnosing trichinella can be problematic because fever and myalgia are nonspecific, and diagnosis may be delayed. We describe 5 Vietnamese patients in whom trichinellosis was diagnosed after several weeks of illness. © 2009 by the Infectious Diseases Society of America. All rights reserved.
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    Development of a blocking latex agglutination test for the detection of antibodies to chicken anemia virus
    (2015-05-04) Dai Quang Trinh; Haruko Ogawa; Vuong Nghia Bui; Tham Thi Hong Nguyen; Dulyatad Gronsang; Tugsbaatar Baatartsogt; Mugimba Kahoza Kizito; Mohammed AboElkhair; Shigeo Yamaguchi; Viet Khong Nguyen; Kunitoshi Imai; Obihiro University of Agriculture and Veterinary Medicine; National Institute of Veterinary Research Hanoi; Mahidol University; Makerere University; The University of Sadat City; Japan Livestock Technology Association
    © 2015 Elsevier B.V. A blocking latex agglutination test (b-LAT) developed in this study was evaluated for the detection of antibodies against chicken anemia virus (CAV) in chickens. Polystyrene latex beads were coupled with a neutralizing monoclonal antibody (mAb) to CAV (mAb-beads). When mAb-beads were mixed with antigens prepared from the lysate of MDCC-MSB1 cells infected with CAV, agglutination occurred. A short pre-incubation of CAV antigens with CAV-specific antiserum inhibited the agglutination of mAb-beads. The test results were obtained within 5. min. The specificity of b-LAT was evaluated using sera from specific pathogen-free chickens and sera containing antibodies to avian influenza virus, Newcastle disease virus, infectious bursal disease virus, and Marek's disease virus; nonspecific agglutination and cross-reactivity with antibodies to unrelated viruses were not observed. The examination of 94 serum samples collected from commercial breeder chickens of various ages (17-63 weeks) revealed good agreement (93.6%, Kappa value. =. 0.82) between b-LAT and a virus neutralization test, known to be most sensitive and specific in the detection of antibodies to CAV. These results indicate that b-LAT, a simple and rapid test, is a useful and reliable tool in CAV serology.