Browsing by Author "Vuthy Sar"

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    The epidemiology of pediatric bone and joint infections in cambodia, 2007-11
    (2013-02-01) Nicole Stoesser; Joanna Pocock; Catrin E. Moore; Sona Soeng; Put Chhat Hor; Poda Sar; Direk Limmathurotsakul; Nicholas Day; Varun Kumar; Sophy Khan; Vuthy Sar; Christopher M. Parry; Angkor Hospital for Children; Mahidol University; University of Oxford; Addenbrooke's Hospital; University of Cambridge
    There are limited data on osteoarticular infections from resource-limited settings in Asia. A retrospective study of patients presenting to the Angkor Hospital for Children, Cambodia, January 2007-July 2011, identified 81 cases (28% monoarticular septic arthritis, 51% single-limb osteomyelitis and 15% multisite infections). The incidence was 13.8/100 000 hospital attendances. The median age was 7.3 years, with a male/female ratio of 1.9:1; 35% presented within 5 days of symptom onset (median 7 days). Staphylococcus aureus was cultured in 29 (36%) cases (52% of culture-positive cases); one isolate was methicillin-resistant (MRSA). Median duration of antimicrobial treatment was 29 days (interquartile range 21-43); rates of surgical intervention were 96%, and 46% of children had sequelae, with one fatality. In this setting osteoarticular infections are relatively common with high rates of surgical intervention and sequelae. Staphylococcus aureus is the commonest culturable cause, but methicillin-resistant S. aureus is not a major problem, unlike in other Asian centers. © The Author [2012]. Published by Oxford University Press. All rights reserved.
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    Panton-valentine leucocidin is the key determinant of staphylococcus aureus pyomyositis in a bacterial GWAS
    (2019-02-01) Bernadette C. Young; Sarah G. Earle; Sona Soeng; Poda Sar; Varun Kumar; Songly Hor; Vuthy Sar; Rachel Bousfield; Nicholas D. Sanderson; Leanne Barker; Nicole Stoesser; Katherine R.W. Emary; Christopher M. Parry; Emma K. Nickerson; Paul Turner; Rory Bowden; Derrick Crook; David Wyllie; Nicholas P.J. Day; Daniel J. Wilson; Catrin E. Moore; Wellcome Trust Centre for Human Genetics; University of Oxford; Liverpool School of Tropical Medicine; Cambridge University Hospitals NHS Foundation Trust; Mahidol University; Nagasaki University; Nuffield Department of Clinical Medicine; John Radcliffe Hospital; East Tennessee State University; Angkor Hospital for Children
    © 2019, eLife Sciences Publications Ltd. All rights reserved. Pyomyositis is a severe bacterial infection of skeletal muscle, commonly affecting children in tropical regions, predominantly caused by Staphylococcus aureus. To understand the contribution of bacterial genomic factors to pyomyositis, we conducted a genome-wide association study of S. aureus cultured from 101 children with pyomyositis and 417 children with asymptomatic nasal carriage attending the Angkor Hospital for Children, Cambodia. We found a strong relationship between bacterial genetic variation and pyomyositis, with estimated heritability 63.8% (95% CI 49.2-78.4%). The presence of the Panton-Valentine leucocidin (PVL) locus increased the odds of pyomyositis 130-fold (p=10- 17.9 ). The signal of association mapped both to the PVL-coding sequence and the sequence immediately upstream. Together these regions explained over 99.9% of heritability (95% CI 93.5-100%). Our results establish staphylococcal pyomyositis, like tetanus and diphtheria, as critically dependent on a single toxin and demonstrate the potential for association studies to identify specific bacterial genes promoting severe human disease.
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    Pediatric suppurative parotitis in cambodia between 2007 and 2011
    (2012-08-01) Nicole Stoesser; Joanna Pocock; Catrin E. Moore; Sona Soeng; Hor P. Chhat; Poda Sar; Direk Limmathurotsakul; Nicholas Day; Vann Thy; Vuthy Sar; Christopher M. Parry; Angkor Hospital for Children; Mahidol University; University of Oxford; Addenbrooke's Hospital; University of Cambridge
    The causes of suppurative parotitis in Cambodian children are not known. We describe 39 cases at the Angkor Hospital for Children, Siem Reap, between January 2007 and July 2011 (0.07/1000 hospital attendances). The median age was 5.7 years with no neonates affected. Burkholderia pseudomallei was cultured in 29 (74%) cases. No deaths occurred; 1 child developed facial nerve palsy. © 2012 by Lippincott Williams & Wilkins.