Browsing by Author "Wasana Jiamtom"

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    Induction of TNF-α in human macrophages by avian and human influenza viruses
    (2010-06-09) Yuwarat Monteerarat; Saori Sakabe; Somying Ngamurulert; Sirawat Srichatraphimuk; Wasana Jiamtom; Kridsada Chaichuen; Arunee Thitithanyanont; Parichart Permpikul; Taweesak Songserm; Pilaipan Puthavathana; Chairul A. Nidom; Le Quynh Mai; Kiyoko Iwatsuki-Horimoto; Yoshihiro Kawaoka; Prasert Auewarakul; Mahidol University; Institute of Medical Science The University of Tokyo; Kasetsart University; Airlannga University; National Institute of Hygiene and Epidemiology Hanoi; University of Wisconsin Madison, School of Veterinary Medicine; Japan Science and Technology Agency
    The highly pathogenic avian influenza virus H5N1 is known to induce high level of tumor necrosis factor α (TNF-α) from primary macrophages. However, it is still unclear whether current H5N1 strains also induce high TNF-α production, as most of the data were derived from extinct clade 0 H5N1 strain. Here, we show that current clade 1 and 2 H5N1 strains induce variable levels of TNF-α that are not necessarily higher than those induced by seasonal influenza viruses. The result suggests that hyper-induction of TNF-α in human macrophages is not always associated with a highly pathogenic phenotype. We further tested the contribution of the NS gene segment from H5N1 isolates to TNF-α induction by using reverse genetics. While NS conferred some variation in TNF-α induction when incorporated into an H1N1 virus genetic background, it did not affect TNF-α induction in an H5N1 virus genetic background, suggesting that other viral genes are involved. © 2010 Springer-Verlag.