Journal Issue: JAAS Vol. 5 No. 2
Issued Date
2555
Resource Type
Language
tha
eng
eng
File Type
application/pdf
Access Rights
open access
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ผลงานนี้เป็นลิขสิทธิ์ของมหาวิทยาลัยมหิดล ขอสงวนไว้สำหรับเพื่อการศึกษาเท่านั้น ต้องอ้างอิงแหล่งที่มา ห้ามดัดแปลงเนื้อหา และห้ามนำไปใช้เพื่อการค้า
Rights Holder(s)
คณะสัตวแพทยศาสตร์ มหาวิทยาลัยมหิดล
Journal Volume
JAAS Volume 5
(2555)
Articles
The importance of epigenetics in embryonic development and reproductive biotechnology
(2012) Tuempong Wongtawan; Mahidol University. Faculty of Veterinary Science. Department of Pre-clinic and Applied Animal Science
It is well established that epigenetics and chromatin modifications are primary factors that can govern
gene activity and nuclear architecture. They are also proven to be essential for normal embryonic development
and cell differentiation. One important step during mouse development is the establishment of epigenetic
reprogramming which is believed to be vital for normal growth and development, however; the mechanism is
still poorly understood. Creating embryo using reproductive biotechnology such as IVM/IVF, ICSI and nuclear
transfer may cause abnormal epigenetic pattern. This review summarise the role and the importance of
epigenetic in embryonic development as well as reproductive biotechnology from research literature mainly
in mouse used as a model for mammalian development.
Effects of feeding chitooligosaccharide on growth performance, immunity and serum composition in goats
(2012) Chowalit Nakthong; Sarawut Taksinoros; Witsanu Wongsawaong; Mahidol University. Faculty of Veterinary Science
A total of 15 female goats (28.5 ± 0.5 kg) were employed to determine the effects of feeding commercial
prebiotic (Nuclear C.O.S), a type of chitooligosaccharide (COS) on growth performance, immunity and serum
composition. A complete randomized design (CRD) was used in the experiment. Goats were randomly allotted into
3 treatments with 5 replications each treatment (a goat/pen). Diets were formulated to meet or exceed nutrient
requirements (NRC, 1998) and COS (Nuclear C.O.S) was added with 3 levels; 0 ppm (control), 1 ppm (LL) and 2
ppm (HL). Blood samples were collected all goats each treatment and four times on day 0, 21, 42 and 63 after
feeding. Goats were bled via venipuncture from jugular vein. During treatment periods, there were trends to
improve weight gain and average daily gain (ADG), as COS (Nuclear C.O.S) levels were increased in the diets,
although it was not statistically significant (p>0.05). The other hand, FCR was reduced when COS (Nuclear C.O.S)
level LL as compared to control level (p>0.05). Cholesterol level in blood was decreased significantly (p<0.05)
although total protein was significantly difference increased in COS (Nuclear C.O.S) treated groups (p<0.05).
There were trend towards reducing triglyceride and trend to upward HDL of goats as dietary COS (Nuclear
C.O.S) was increased (p>0.05). During the overall period, adding high level (HL) improved lymphocyte cell
count (p>0.05). White blood cell was not significantly reduced when added level of COS (Nuclear C.O.S)
(p>0.05). In conclusion, nutrients would be utilized more efficiently in goats fed diet containing COS
(Nuclear C.O.S) than those fed control diets due to better condition of microbial populations in alimentary tracts.
Preliminary report: Homology Modeling of human, dog and catβ-adrenergic receptors
(2012) Waraphan Toniti; Pranom Puchadapirom; Aekkapot Chamkasem; Tawewan Tansatit; Mahidol University. Faculty of Veterinary Science. Department of Pre-clinic and Applied Animal Sciences; Mahidol University. Faculty of Science. Department of Pathobiology
Beta 2-adrenergic Receptors are widely distributed in heart, airway smooth muscle, liver, skeletal
muscle and adipose tissue. The specific ligand, beta 2-agonist, binds at the specific binding site on ADRB2 then
the signal transduction pathways begins. The three-dimensional configuration of ADRB2 is uniquely and
determines by its amino acid sequence. In this study, human ADRB2, dog ADRB2, and cat ADRB2 sequences
were studied by sequence of P07550, P54833 and Q9TST5, respectively. The similarity of human ADRB2 and
dog ADRB2 was 90% whereas the similarity between human ADRB2 and cat ADRB2 was 89%. In addition,
the similarity between dog ADRB2 and cat ADRB2 was 95%. The in silico ADRB2 models were generated
and compared among species of interest. The results show that the shapes and the pocket sites of ADRB2
differ between species. The differences of ADRB2 three-dimensional configuration may give explanations about
the variety of interaction between ADRB2 and its agonist among human, dog and cat. Further study of
protein-protein interaction, structure-based drug design, and novel function of ADRB2 in companion animals
may have basis from human ADRB2.
การพัฒนา Diploid cells จากเซลล์เหงือกของปลาคาร์พ เพื่อใช้ในการแยกเชื้อไวรัสคอยเฮอร์ปีส์
(2555) น้ำอ้อย เถาวัลย์; นัทธภัทร เกตุฉิม; สมจิตร ใช้วัฒนรุ่งเรืองไพศาล; ปรุศก์ สุกใส; แพรวพร ไทยจงรักษ์; กฤษฎา ใจชื้น; มหาวิทยาลัยมหิดล. คณะสัตวแพทยศาสตร์. ศูนย์เฝ้าระวังและติดตามโรคจากสัตว์ป่า สัตว์ต่างถิ่นและสัตว์อพยพ; มหาวิทยาลัยมหิดล. คณะสัตวแพทยศาสตร์. ภาควิชาเวชศาสตร์คลินิกและการสาธารณสุข; มหาวิทยาลัยมหิดล. คณะสัตวแพทยศาสตร์. ภาควิชาปรีคลินิกและสัตวศาสตร์ประยุกต์
Koi herpesvirus (KHV) is a cause of morbidity and mortality in fancy carp (koi; Cyprinus carpio koi)
and common carp (Cyprinus carpio). The development of diploid cells for viral isolation is important for
laboratory diagnosis and research. In this study, KHV was cultured consecutively in koi gill (KG) cells of
passage 29 - 30 and 33 - 34. The fluid from each passage was tested for KHV by polymerase chain reaction
technique and positive result was found in both passages. Thus, KG cells can provide a positive result to
KHV isolation at 25oC and this can be confirmed by PCR.
Title
JAAS Vol. 5 No. 2
Author's Affiliation
มหาวิทยาลัยมหิดล. คณะสัตวแพทยศาสตร์