Publication: Supplemental effect of varying L-cysteine concentrations on the quality of cryopreserved boar semen.
Issued Date
2010
Resource Type
Language
eng
ISSN
1745-7262
DOI
Rights
Mahidol University
Rights Holder(s)
AJA, SIMM & SJTU
Bibliographic Citation
Asian Journal of Andrology. Vol.12, No.5 (Sep 2010), 760-765
Suggested Citation
Kampon Kaeoket, Panida Chanapiwat, Padet Tummaruk, Mongkol Techakumphu Supplemental effect of varying L-cysteine concentrations on the quality of cryopreserved boar semen.. Asian Journal of Andrology. Vol.12, No.5 (Sep 2010), 760-765. doi:10.1038/aja.2010 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/1645
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Supplemental effect of varying L-cysteine concentrations on the quality of cryopreserved boar semen.
Other Contributor(s)
Abstract
Cryopreservation is associated with the production of reactive oxygen species, which leads to lipid peroxidation of the sperm membrane and consequently a reduction in sperm motility and decreased fertility potential. The aim of this study was to determine the optimal concentration of L-cysteine needed for cryopreservation of boar semen. Twelve boars provided semen of proven motility and morphology for this study. The semen was divided into four portions in which the lactose-egg yolk (LEY) extender used to resuspend the centrifuged sperm pellet was supplemented with various concentrations of L-cysteine to reach 0 mmol L−1(group I, control), 5 mmol L−1(group II), 10 mmol L−1(group III) and 15 mmol L−1(group IV). Semen suspensions were loaded in straws (0.5 mL) and placed in a controlled-rate freezer. After cryopreservation, frozen semen samples were thawed and investigated for progressive motility, viability using SYBR-14/EthD-1 staining and acrosome integrity using FITC-PNA/EthD-1 staining. There was a significantly higher (P < 0.01) percentage of progressive motility, viability and acrosomal integrity in two L-cysteine-supplemented groups (group II and group III) compared with the control. There was a biphasic effect of L-cysteine, with the highest percentage of progressive motility, viability and acrosomal integrity in group III. In
conclusion, 5 or 10 mmol L−1 was the optimum concentration of L-cysteine to be added to the LEY extender for improving the quality of frozen–thawed boar semen.