Publication: Immunohistochemical study of acute and chronic toxoplasmosis in experimentally infected mice.
Issued Date
2007
Resource Type
Language
eng
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Mahidol University
Rights Holder(s)
Faculty of Tropical Medicine
Bibliographic Citation
Southeast Asian Journal of Tropical Medicine and Public Health. Vol. 38, No. 2 (2007), 223-31
Suggested Citation
Waree, P., Fergusson, D.J., Pongponratn, E., Chaisri, U., Sukthana, Y. Immunohistochemical study of acute and chronic toxoplasmosis in experimentally infected mice.. Southeast Asian Journal of Tropical Medicine and Public Health. Vol. 38, No. 2 (2007), 223-31. Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/9903
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Title
Immunohistochemical study of acute and chronic toxoplasmosis in experimentally infected mice.
Abstract
Acute and chronic Toxoplasma infections were evaluated in mice using stage
specific antibodies and immunocytochemistry. Mice with acute toxoplasmosis
were less active, had erectile body hair and seldom took food or water resulting
in weight loss. All mice died within 7 days post-inoculation. The
immunohistochemical technique enhanced visualization of parasites allowing
their distribution to be accurately followed. Following intraperitoneal infection,
tachyzoites were initially identified on the surface of the liver and spleen. There
was a rapid increase in the number of tachyzoites associated with invasion from
the surrounding connective tissue into the organs with formation of
inflammatory lesions in the liver. The focal inflammatory lesions showed
increasing numbers of tachyzoites with the period post-inoculation. Similar
increases in tachyzoites were observed for the spleen. In contrast, only a few
individual tachyzoites were seen in the brain at the final time point. In chronic
infections, the mice were asymptomatic but tissue cysts containing large
numbers of bradyzoites were observed in all brains with the average number of
295 tissue cysts per half brain and the average cystic size of 46.02 +/- 5.08
microm. By histology and immunostaining, the tissue cysts were readily
identifiable along with a mild inflammatory cell infiltration into the meninges
and perivascular cuffing. Double immunocytochemical labelling confirmed the
exclusive presence of tachyzoites during the acute phase and bradyzoites during
the chronic phase.