Publication: Prevalence and genotypic relatedness of carbapenem resistance among multidrug-resistant P. aeruginosa in tertiary hospitals across Thailand
Issued Date
2012
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eng
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Mahidol University
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BioMed Central
Bibliographic Citation
Annals of Clinical Microbiology and Antimicrobials. 11, (2012), 25
Suggested Citation
Piyatip Khuntayaporn, Preecha Montakantikul, Piroon Mootsikapun, Visanu Thamlikitkul, Mullika Traidej Chomnawang Prevalence and genotypic relatedness of carbapenem resistance among multidrug-resistant P. aeruginosa in tertiary hospitals across Thailand. Annals of Clinical Microbiology and Antimicrobials. 11, (2012), 25. doi:https://doi.org/10.1186/1476-0711-11-25 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/2775
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Title
Prevalence and genotypic relatedness of carbapenem resistance among multidrug-resistant P. aeruginosa in tertiary hospitals across Thailand
Abstract
Background: Increased infection caused by multidrug resistant (MDR) Pseudomonas aeruginosa has raised
awareness of the resistance situation worldwide. Carbapenem resistance among MDR (CR-MDR) P. aeruginosa has
become a serious life-threatening problem due to the limited therapeutic options. Therefore, the objectives of
this study were to determine the prevalence, the antibiotic susceptibility patterns and the relatedness of CR-MDR
P. aeruginosa in tertiary hospitals across Thailand.
Methods: MDR P. aeruginosa from eight tertiary hospitals across Thailand were collected from 2007–2009.
Susceptibility of P. aeruginosa clinical isolates was determined according to the Clinical and Laboratory Standards
Institute guideline. Selected CR-MDR P. aeruginosa isolates were genetically analyzed by pulsed-field gel
electrophoresis.
Results: About 261 clinical isolates were identified as MDR P. aeruginosa and approximately 71.65% were found
to be CR-MDR P. aeruginosa. The result showed that the meropenem resistance rate was the highest reaching
over 50% in every hospitals. Additionally, the type of hospitals was a major factor affecting the resistance rate,
as demonstrated by significantly higher CR-MDR rates among university and regional hospitals. The fingerprinting
map identified 107 clones with at least 95% similarity. Only 4 clones were detected in more than one hospital.
Conclusions: Although the antibiotic resistance rate was high, the spreading of CR-MDR was found locally.
Specific strains of CR-MDR did not commonly spread from one hospital to another. Importantly, clonal
dissemination ratio indicated limited intra-hospital transmission in Thailand.