Publication: Antiosteoporotic effect of sequential extracts and freezedried juice of Cissus quadrangularis L. in ovariectomized mice
Issued Date
2012
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eng
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Mahidol University
Bibliographic Citation
Asian Biomedicine. Vol. 6, No. 3 (June 2012), 377 - 384
Suggested Citation
Thanika Pathomwichaiwat, Wisuda Suvitayavat, Achariya Sailasuta, Pawinee Piyachaturawat, Noppamas Soonthornchareonnon, Sompop Prathanturarug Antiosteoporotic effect of sequential extracts and freezedried juice of Cissus quadrangularis L. in ovariectomized mice. Asian Biomedicine. Vol. 6, No. 3 (June 2012), 377 - 384. doi:10.5372/1905-7415.0603.069 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/2620
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Title
Antiosteoporotic effect of sequential extracts and freezedried juice of Cissus quadrangularis L. in ovariectomized mice
Abstract
Background: Osteoporosis becomes a major health problem in aging populations, and this disease increases
the risk of bone fractures, leading to disability and mortality. Cissus quadrangularis L. (CQ) has been reported
to have beneficial effects on bone metabolism; however, there has been no investigation to identify the active
compounds responsible for this activity.
Objective: Sequential extracts (hexane, dichloromethane, ethanol, and water) and freeze-dried CQ juice were
investigated to determine their effects on bone metabolism in an ovariectomized (ovx) mouse model.
Methods: Six-week-old ICR mice were divided into eight groups: sham-operated, ovx-control, estradiol (E2)-
treated and five CQ-treated ovx-mouse groups (n = 3). The CQ extracts were orally administered at a dose
equivalent to 5g of crude powder/kg/day for 8 weeks. Bone mineral densities (BMD) of the femur and tibia,
serum levels of osteocalcin (bone formation marker) and TRAP5b (bone resorption marker), and histomorphological
change of lumbar spine were determined at the end of the experiment.
Results: The BMD of the femur and tibia in the hexane-treated group were elevated to the same level as those
of sham-operated group. This BMDs correlated with restoration of the trabecular bone of the lumbar spine,
which was only observed in the hexane-treated group. These results were also supported by the lowest serum
levels of osteocalcin and TRAP5b observed in this group, compared to the ovx-control and E2-treated groups,
representing a decrease in the bone turnover rate. Neither signs of abnormality nor pathological changes of
internal organs were observed after the experiment.
Conclusion: The hexane extract possessed antiosteoporotic activity in ovariectomized mice without any toxicity
throughout the experiment. Therefore, the hexane extract is the most interesting for further bioassay-guided
purification of pharmacologically active compounds.