Publication: Quality and quantity of Asian elephant (Elephas maximus) peripheral blood mononuclear cells isolated by polymer of sucrose or colloidal silica solution gradient centrifugation
Issued Date
2008
Resource Type
Language
eng
ISSN
1906-2257
Rights
Mahidol University
Rights Holder(s)
Faculty of Veterinary Science Mahidol University
Bibliographic Citation
Journal of Applied Animal Science. Vol.1, No.1 (2008), 31-38
Suggested Citation
Porntep Gittipongsiri, Somphon Makpun, Teeraphong Phansod, Dulyatad Gronsang, Roschong Boonyarittichaikij, Witthawat Wiriyarat Quality and quantity of Asian elephant (Elephas maximus) peripheral blood mononuclear cells isolated by polymer of sucrose or colloidal silica solution gradient centrifugation. Journal of Applied Animal Science. Vol.1, No.1 (2008), 31-38. Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/11023
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Title
Quality and quantity of Asian elephant (Elephas maximus) peripheral blood mononuclear cells isolated by polymer of sucrose or colloidal silica solution gradient centrifugation
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Abstract
Peripheral blood mononuclear cells (PBMCs) are useful in cellular immunology, infectious disease
diagnosis and molecular genetics research. This study was aimed to determine an appropriated procedure for
PBMCs isolation from an Asian elephant (Elephas maximus) by using gradient centrifugations method. The
efficacy of four different density solutions including polymer of sucrose solution (Ficoll-Hypaque mixture) of
density 1.077 and colloidal silica solution (Percoll) of density 1.077, 1.078 and 1.079 g/cm3 were compared in
elephant PBMC separation. Quality and quantity of isolated PBMCs were estimated by using five parameters
including cell purity, cell recovery rate, cell viability, viability of PBMCs after 5 days cultivation and total cell
count. The results showed that the total cell count was significantly different among 4 treatments, in which
Percoll with density 1.079 g/cm3 yielded the highest PBMCs (average = 3.94 X 107 cells/cm3) and highest PBMCs
recovery rate (average = 82.61%). However, the purity, viability and viability of PBMCs after 5 days cultivation
were not significantly different (P<0.05). In conclusion, Percoll with a density of 1.079 g/cm3 is the most suitable
density gradient treatment for Asian elephant PBMCs isolation in both quantity and quality aspects.