Publication:
In vivo culture of cryptosporidium oocysts for laboratory use.

Issued Date

2006

Resource Type

Article

Language

eng

Rights

Mahidol University.

Bibliographic Citation

Southeast Asian Journal Tropical Medicine Public Health. Vol.37 (Suppl. 3), (2006), 18-20

Suggested Citation

Sangloung, C., Buddhirongawatr, R., Tantawiwattananon, N., Sukthana, Y., Charoonluk Sangloung., Nitipan Tantawiwattananon., Ruangrat Buddhirongawatr., Yaowalark Sukthana. In vivo culture of cryptosporidium oocysts for laboratory use.. Southeast Asian Journal Tropical Medicine Public Health. Vol.37 (Suppl. 3), (2006), 18-20. Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/8779

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Title

In vivo culture of cryptosporidium oocysts for laboratory use.

Author(s)

Sangloung, C.
 Buddhirongawatr, R.
 Tantawiwattananon, N.
 Sukthana, Y.
 Charoonluk Sangloung.
 Nitipan Tantawiwattananon.
 Ruangrat Buddhirongawatr.
 Yaowalark Sukthana.

Other Contributor(s)

Mahidol University. International College. Planning Research and Development Department.

Abstract

In the present study, we describe in vivo cultivation to produce oocysts. Seven-day-old mice were orally infected with 100,00120,000 Cryptosporidium oocysts. On day 8 post-infection, the mice were killed by ether, and the small and large intestines collected. A simple extraction procedure was used and purified using Ficoll gradient centrifugation. After purification, the oocysts were preserved in phosphate buffered saline with antibiotic at 4°C before use.

Keyword(s)

Cryptosporidium culture.
 Animal model.

Availability

URI

https://repository.li.mahidol.ac.th/handle/20.500.14594/8779

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MUIC-Article