Publication: Micronucleus test : the effect of ascorbic acid on cadmium exposure in fish (puntius altus).
Issued Date
2007
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eng
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Mahidol University
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Bibliographic Citation
Reserch Journal of Environmental Toxicology. Vol. 1, No. 1 (2007), 27-36
Suggested Citation
Wanne Jiraungkoorskul., Somphong Sahaphong., Piya Kosai., Myung-Huk Kim. Micronucleus test : the effect of ascorbic acid on cadmium exposure in fish (puntius altus).. Reserch Journal of Environmental Toxicology. Vol. 1, No. 1 (2007), 27-36. Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/9952
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Title
Micronucleus test : the effect of ascorbic acid on cadmium exposure in fish (puntius altus).
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Abstract
Micronucleus formation in fish cells, as an indicator of chromosomal damage,
has been increasingly used to detect the genotoxic potential of heavy metal
exposure. This study was investigated the effects of cadmium (Cd) and ascorbic
acid (AA) on the red-tailed tinfoil barb (Puntius altus) using the micronucleus
(MN) and nuclear abnormality (NA) tests for the period of 24, 48, 72 and 96 h.
The MN frequencies in the erythrocytes, gill, liver and fin cells were analyzed
comparatively to evaluate the sensitivity and suitability of these different cell
types. NA shapes in erythrocytes were scored into blebbed nuclei (BL), lobed
nuclei (LB), notched nuclei (NT) and binuclei (BN). It was observed that, fish
showed significant sensitivity to the different treatments. In general, the highest
value of both MN and NA cells were significantly increased in the Cd treated
group followed by the combination of Cd and AA treated group. On the other
hand, the MN and NA frequencies in erythrocytes were the most sensitive to the
treatment and could provide valuable information than those in gill, liver and fin
cells. The frequencies of each NA shape in erythrocytes of all treatments were
observed in the following NT > LB > BN > BL. Results of MN and NA
revealed the highest number after 48 h treatment in all cases and decreased
within the longer time exposure. Our results demonstrated the efficacy of AA in
reducing genotoxicity in fish induced by Cd. Otherwise; they showed the
sensitivity and suitability of MN and NA frequencies in erythrocytes for
pollution biomarkers.