Publication: Efficacy of cinnamon bark oil and cinnamaldehyde on anti-multidrug resistant Pseudomonas aeruginosa and the synergistic effects in combination with other antimicrobial agents
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2016
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eng
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Mahidol University
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BioMed Central
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BMC Complementary and Alternative Medicine. Vol.16, (2016),158
Suggested Citation
Itsaraporn Utchariyakiat, Suvimol Surassmo, Montree Jaturanpinyo, Piyatip Khuntayaporn, Mullika Traidej Chomnawang Efficacy of cinnamon bark oil and cinnamaldehyde on anti-multidrug resistant Pseudomonas aeruginosa and the synergistic effects in combination with other antimicrobial agents. BMC Complementary and Alternative Medicine. Vol.16, (2016),158. doi:10.1186/s12906-016-1134-9 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/2844
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Efficacy of cinnamon bark oil and cinnamaldehyde on anti-multidrug resistant Pseudomonas aeruginosa and the synergistic effects in combination with other antimicrobial agents
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Abstract
Background: The emergence of drug resistant pathogens becomes a crucial problem for infectious diseases worldwide.
Among these bacteria, Pseudomonas aeruginosa is one of which highly resists to many currently used drugs
and becomes a major concern in public health. Up till now, the search for potential antimicrobial agents has
been still a challenge for researchers.
Methods: Broth microdilution assay was used to determine minimum inhibitory concentrations (MICs) and
minimum bactericidal concentrations (MBCs) of the essential oils and antibiotics against P. aeruginosa.
Inhibition activity of the essential oils under vapor condition was examined to obtain the minimum inhibitory
dose (MID). Time-kill assay included in this study was performed according to CLSI guideline. Bioautographic assay was
used to detect active components of the essential oil. Synergistic effect with currently used antibiotics was further
examined by checkerboard assay.
Results and Discussion: In this study, a variety of essential oils were examined for anti-multidrug resistant P.
aeruginosa (MDR-PA) activity, of which cinnamon bark oil showed the strongest antimicrobial activity against
all clinical-isolated MDR-PA strains with MIC of 0.0562–0.225 % v/v and MBC of 0.1125–1.8 % v/v.
Bioautographic results demonstrated that the active compounds of cinnamon bark oil were cinnamaldehyde and
eugenol which showed strong inhibitory effect against P. aeruginosa. Interestingly, cinnamaldehyde, a major
constituent of cinnamon bark oil, possessed stronger antimicrobial effect to P. aeruginosa than eugenol. Under gaseous
condition, cinnamon bark oil and cinnamaldehyde showed antibacterial activity against MDR-PA strains with MID of 0.
5–1 mg/L. Moreover, combination of cinnamon bark oil or cinnamaldehyde with currently used antibiotics was
further studied by checkerboard assay to examine synergistic interactions on clinically isolated MDR-PA strains.
Cinnamon bark oil and cinnamaldehyde combined with colistin demonstrated synergistic rates at 16.7 and
10 %, respectively.
Conclusion: These results indicated that cinnamon bark oil and cinnamaldehyde might be active natural compounds
which could be further examined as alternative treatment for multidrug-resistant P. aeruginosa infection.