Publication:
In vitro study of the SBFand osteoblast-like cells on hydroxyapatite/chitosan–silica nanocomposite

dc.contributor.authorPrapaporn Jongwattanapisanen_US
dc.contributor.authorNarattaphol Charoenphandhuen_US
dc.contributor.authorNateetip Krishnamraen_US
dc.contributor.authorJirawan Thongbunchooen_US
dc.contributor.authorI-Ming Tangen_US
dc.contributor.authorRassmidara Hoonsawaten_US
dc.contributor.authorSiwaporn Meejoo Smithen_US
dc.contributor.authorWeeraphat Pon-Onen_US
dc.contributor.otherMahidol University. Mahidol University International College. Science Division.en_US
dc.date.accessioned2015-09-04T08:35:26Z
dc.date.accessioned2018-04-17T14:35:15Z
dc.date.available2015-09-04T08:35:26Z
dc.date.available2018-04-17T14:35:15Z
dc.date.created2015
dc.date.issued2011
dc.description.abstractHydroxyapatite/chitosan-silica (HApCSi) nanocomposites were synthesized by co-precipitated method and their potential application as filler materials for bone regeneration were investigated in simulated body fluid (SBF). To study their biocompatibility, they were cultured with rat osteoblast-like UMR-106 cells for 3, 7, 14, and 21 days. Studies of the silica contents in chitosan matrix showed the presence of silinol (Si-OH) groups in CSi hybrid and their decrease after being composited with calcium phosphate (CaP) which is desirable for the formation of the apatite. XRD and TEM studies showed that the HAp formed in the CSi matrix were nanometer (20-40 nm) in size. anocomposites of HApCSi20 processed with 20%v/v silica whisker showed a micro hardness of 84.7 ± 3.3 MPa. Mineralization study in SBF showed the formation of apatite crystals on the HApCSi surface after being incubated for 7 days. In vitro biocompatibility, cell morphology, proliferation, and cell adhesion tests confirmed the osteoblast attachment and growth on the HApCSi20 surface. The density of cells and the production of calcium nodules on the substrate were seen to increase with increasing cultured time. The mechanical evaluation and in vitro experiment suggested that the use of HApCSi composite will lead to the formation of new apatite particles and thus be a potential implant material.en_US
dc.identifier.citationMaterials Science and Engineering C. Vol. 31, No. 2 (2011), 290-299en_US
dc.identifier.urihttps://repository.li.mahidol.ac.th/handle/20.500.14594/10540
dc.language.isoengen_US
dc.rightsMahidol Universityen_US
dc.rights.holderScienceDirecten_US
dc.subjectBiocompositesen_US
dc.subjectCell adhesionen_US
dc.subjectChitosanen_US
dc.subjectHydoxyapatiteen_US
dc.subjectOsteoblasten_US
dc.titleIn vitro study of the SBFand osteoblast-like cells on hydroxyapatite/chitosan–silica nanocompositeen_US
dc.typeArticleen_US
dspace.entity.typePublication
mods.location.urlhttp://www.sciencedirect.com/science/article/pii/S0928493110002274

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