Giardia and cryptosporidium as biohazard in frozen food products.
5
Issued Date
2007
Resource Type
Language
eng
Rights
Mahidol University
Suggested Citation
Yaowalark Sukthana. (2007). Giardia and cryptosporidium as biohazard in frozen food products.. Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/35023
Title
Giardia and cryptosporidium as biohazard in frozen food products.
Author(s)
Abstract
Giardia and Cryptosporidium commonly cause food and water-borne disease
outbreaks worldwide. They occur in both normal and immunocompromised
hosts causing both health and economic burdens. The globalization of Free
Trade Agreement (FTA) on food products increases the possibility of more
extensive transmission. Hazard Analysis Critical Control Point (HACCP)
system at present covers only conventional bacteria. As a major seafood
exporter to various regions of the world, Thailand is considering to include
those protozoa into the HACCP for the frozen food industries.
Hazard identification of protozoa was performed in raw materials, food
products and water used in the industry. Raw and treated water samples were
collected by large volume technique through a filter with 1 micrometer (μm)
nominal porosity. Immunomagnetic separation (IMS) for Giardia and
Cryptosporidium was then applied to eluted and concentrated water.
Identifications were individually performed by immunofluorescent and PCR
techniques. In raw water, Giardia was found in 42.8-60% whilst
Cryptosporidium was found in 20-35%, but no contamination in treated water
samples was found. Giardia was also identified in raw materials and food
products. The viability of recovered protozoa was analyzed by the reverse
transcriptase polymerase chain reaction (RT-PCR). The giardin beta-subunit
mRNA gene was selected as the target. Positive RT-PCR for Giardia recovered
from raw materials and food products indicates viability. The effect of various
concentrations of ultra violet light and difference in duration of solar exposure
to control those viable recovered biohazards was studied.
Description
Giardia and Crytosporidium Conference, Mexico. May 13-18, 2007