Investigation of possible interference of a metabolite of etoricoxib (Arcoxia®) in the detection of 16a-hydroxyprednisolone by LC-MS/MS
Issued Date
2014
Resource Type
Language
eng
Rights
Mahidol University
Rights Holder(s)
National Doping Control Centre Mahidol University
Suggested Citation
Saardpun N, Panan S, Wilairat P, Chaikum N (2014). Investigation of possible interference of a metabolite of etoricoxib (Arcoxia®) in the detection of 16a-hydroxyprednisolone by LC-MS/MS. Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/44212
Title
Investigation of possible interference of a metabolite of etoricoxib (Arcoxia®) in the detection of 16a-hydroxyprednisolone by LC-MS/MS
Author(s)
Other Contributor(s)
Abstract
Etoricoxib (Arcoxia®) is a recent drug for the effective treatment of pain, inflammation and fever. It is available without
prescription in Thailand but may require a physician’s prescription in other countries. This investigation was initiated by the
presence of a large peak in about 10% of the MRM chromatograms of our screening procedure for corticosteroids by
LC-MS/MS. This peak was in the time window of the MRM transition for 16a-hydroxyprednisolone. Fortunately for some of
these samples the data sheet for previous use of medication stated the use of Arcoxia®.
To confirm that the interfering peak was due to a metabolite of the drug etoricoxib (Arcoxia®) an excretion study of a male
volunteer who had taken a 30-mg single oral dose of etoricoxib was performed. Metabolites of the drug were identified by
comparison of the observed mass spectra (both full scan and product ion scan) with that reported in the literature. It was
shown that the interference peak was 6’-hydroxymethyl-etoricoxib. The mass spectrum information of this metabolite was
then used to modify the MRM transition for 16a-hydroxyprednisolone in the screening method.
Description
Recent Advances in Doping Analysis (21) Proceedings of the Manfred Donike Workshop 31th Cologne Workshop on Dope Analysis 24th February to 1st March 2013, Page 117-120