Detection of deacetylmetipranolol arising from storage of standard solution of metipranolol
2
Issued Date
2015
Resource Type
Language
eng
Rights
Mahidol University
Rights Holder(s)
National Doping Control Centre Mahidol University
Suggested Citation
Inthong T, Matayatsuk Phechkrajang C, Wilairat P, Chaikum N (2015). Detection of deacetylmetipranolol arising from storage of standard solution of metipranolol. Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/44137
Title
Detection of deacetylmetipranolol arising from storage of standard solution of metipranolol
Other Contributor(s)
Abstract
Metipranolol is a beta-blocker in the WADA list of substances prohibited in particular sports. It is converted to deacetylmetipranolol by the first pass metabolism and the parent compound is not excreted. A standard methanolic solution of metipranolol can be converted to deacetylmetipranolol during prolonged storage at -20°C. Spiking blank urine with this aged standard can lead to misinterpretation of the form of metipranolol in a suspected urine sample since the normal GC-MS screening procedure for derivatized beta-blockers cannot separate this metabolite from the parent compound. A specific Ge-temperature program is required. However a LC-MS/MS method can separate metipranolol and its metabolite.
Description
Recent advances in doping analysis (22) : Proceedings of the Manfred-Donike-Workshop ; 32nd Cologne Workshop on Dope Analysis ; 30th March to 4th April 2014, page 79-83