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Publication Metadata only The existence of opioid receptors in the cochlea of guinea pigs(2006-05-01) Nopporn Jongkamonwiwat; Pansiri Phansuwan-Pujito; Stefano O. Casalotti; Andrew Forge; Hilary Dodson; Piyarat Govitrapong; The Institute of Science and Technology for Research and Development, Mahidol University; Srinakharinwirot University; UCL; Mahidol Universityof opioid fibers remains to be investigated. In the present study the expression of opioid receptors and their peptides is demonstrated in young adult guinea pig cochlea. Opioid receptors are mainly expressed in hair cells of the organ of Corti and in inner... and outer spiral bundles with different characteristics for each type of receptor. Co-localization studies were employed to compare the distribution of mu-, delta- and kappa-opioid receptors and their respective peptides, β-endorphin, leu-enkephalinPublication Open Access Opioid modulation of GABA release in the rat inferior colliculus(2004) Walaiporn Tongjaroenbungam; Nopporn Jongkamonwiwat; Joanna Cunningham; Pansiri Phansuwan-Pujito; Hilary C Dodson; Andrew Forge; Piyarat Govitrapong; Stefano O Casalottiindicating that micro rather than delta or kappa opioid receptors mediate this action. [3H]GABA release was diminished by 16%, and this was not altered by the protein kinase C inhibitor bisindolylmaleimide I. Immunostaining of inferior colliculus cryosections... shows extensive staining for glutamic acid decarboxylase, more limited staining for micro opiate receptors and relatively few neurons co-stained for both proteins. CONCLUSION: The results suggest that micro-opioid receptor ligands can modifyPublication Metadata only The presence of opioid receptors in rat inner ear(2003-01-01) Nopporn Jongkamonwiwat; Pansiri Phansuwan-Pujito; Puangkeaw Sarapoke; Banthit Chetsawang; Stefano O. Casalotti; Andrew Forge; Hilary Dodson; Piyarat Govitrapong; The Institute of Science and Technology for Research and Development, Mahidol University; Srinakharinwirot University; UCLOpioid peptides have been identified in the inner ear but relatively little information is available about the expression and distribution of their receptors. The aim of the present study was therefore to identify and localize the μ (MOR), δ (DOR...) and κ (KOR) opioid receptor subtypes within the rat cochlea. The expression of these opioid receptor subtypes was determined by reverse transcriptase-polymerase chain reaction followed by nested polymerase chain reaction analysis. Amplification of RNAsPublication Metadata only The opioid receptors in inner ear of different stages of postnatal rats(2003-01-01) Pansiri Phansuwan-Pujito; Ladda Saleema; Sujira Mukda; Walaiporn Tongjaroenbuangam; Nuanchan Jutapakdeegul; Stefano O. Casalotti; Andrew Forge; Hilary Dodson; Piyarat Govitrapong; Srinakharinwirot University; The Institute of Science and Technology for Research and Development, Mahidol University; UCLThere is increasing evidence that the opioid system has a role in hearing. To provide further evidence for such a role, the expression of opioid receptor mRNAs and proteins in the inner ear of rats was studied during development from birth (P0...) to postnatal day 16 (P16). A semi-quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) was employed to detect changes in the expression of delta- (DOR) kappa- (KOR) and mu- (MOR) opioid receptor mRNAs in rat cochleae at P0, P4, P8 and P16Publication Metadata only Opioid modulation of GABA release in the rat inferior colliculus(2004-09-07) Walaiporn Tongjaroenbungam; Nopporn Jongkamonwiwat; Joanna Cunningham; Pansiri Phansuwan-Pujito; Hilary C. Dodson; Andrew Forge; Piyarat Govitrapong; Stefano O. Casalotti; Mahidol University; King's College London; Srinakharinwirot University; UCLindicating that μ rather than δ or κ opioid receptors mediate this action. [3H]GABA release was diminished by 16%, and this was not altered by the protein kinase C inhibitor bisindolylmaleimide I. Immunostaining of inferior colliculus cryosections shows... extensive staining for glutamic acid decarboxylase, more limited staining for μ opiate receptors and relatively few neurons co-stained for both proteins. Conclusion: The results suggest that μ-opioid receptor ligands can modify neurotransmitter release in a