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Item Metadata only Biodegradation of Di-2-Ethylhexyl Phthalate by Mangrove Sediment Microbiome Impacted by Chronic Plastic Waste(2025-02-01) Saeng-kla K.; Mhuantong W.; Termsaithong T.; Pinyakong O.; Sonthiphand P.; Saeng-kla K.; Mahidol UniversityPlastic pollution through the leaching of di(2-ethylhexyl) phthalate (DEHP), a widely used plasticizer, has led to the emergence of mangrove pollution. This study aimed to assess the DEHP removal efficiency of indigenous mangrove sediment... microbiomes and identify key DEHP degraders using microcosm construction and metagenomic analysis. During the 35-day incubation period, the indigenous mangrove sediment microbiome, affected by chronic plastic pollution, demonstrated a 99% degradationItem Metadata only Development of a plastic waste treatment process by combining deep eutectic solvent (DES) pretreatment and bioaugmentation with a plastic-degrading bacterial consortium(2023-10-15) Krainara S.; Mistry A.N.; Malee C.; Chavananikul C.; Pinyakong O.; Assavalapsakul W.; Jitpraphai S.M.; Kachenchart B.; Luepromchai E.; Mahidol Universitywas to develop an effective PET and PLA waste treatment process that involves pretreatment with deep eutectic solvent (DES) followed by biodegradation with a plastic-degrading bacterial consortium in a composting system. The DES used was a mixture of choline... the bacterial consortium were selected based on their ability to biodegrade PET, PLA, and plasticizer. The plastic samples (a PET bottle, PLA cup, and PLA film) were pretreated with DES through a dip-coating method. The DES-coated plastic samples exhibitedItem Metadata only Development of defined bacterial consortium as a bioaugmentation product for degrading mixed plastic wastes and plasticizers in simulated landfill(2025-10-01) Krainara S.; Chavananikul C.; Mistry A.N.; Assavalapsakul W.; Jitpraphai S.M.; Kachenchart B.; Khondee N.; Luepromchai E.; Pinyakong O.; Krainara S.; Mahidol Universitythe levels of EA63 and A9 in an optimized ratio within the consortium improved the degradation efficiency. The effects of temperature, pH, and salinity on plastic and PAE biodegradation were evaluated. At 40 ± 3 °C, the consortium efficiently degraded PLA (31...Plastics and plasticizers pose significant waste management challenges, especially in landfills where degradation is slow. A plastic-degrading bacterial consortium offers a promising solution to accelerate this process. In this study, a bacterialItem Metadata only Unveiling a novel Neobacillus strain: Optimization, metabolomic, and genomic insights into polyvinyl chloride microplastic biodegradation(2026-06-01) Choonut A.; Wongfaed N.; Poolpol A.; Jumpa T.; Boonlue S.; Wantala K.; Pinyakong O.; Reungsang A.; Plangklang P.; Sittijunda S.; Choonut A.; Mahidol UniversityPolyvinyl chloride microplastics (PVC-MPs) are persistent environmental contaminants requiring effective remediation strategies. This study investigated microbial-mediated biodegradation by isolating bacteria from landfill soil samples... with prolonged plastic waste exposure. PVC microplastics (Mn ≈ 35,000 g/mol, Mw ≈ 62,000 g/mol) were used as the substrate. Two microbial consortia, CPVC-KKU2 and CPVC-KKU6, demonstrated acid and lipase production capabilities. Subsequent isolation yielded five