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Item Metadata only Construction of specific DNA probes for Opisthorchis viverrini detection and an attempt to clone genes coding for diagnostic epitopes(Mahidol University. Mahidol University Library and Knowledge Center, 2023) Rasana Sermswan; Stitaya Sirisinha; Sakol Panyim; Skorn Mongkolsukpossess some difficulties due to frequent cross reactivity of O.viverrini antigens with those of other parasites. Although from an antigen analysis, the 89 kDa protein give promising results. Unfortunately, O.viverrini cannot be cultured in vitro which... making it difficult to prepare a substantial quantities of the antigenic proteins. Thus, cloning a gene encoding for some of these specific proteins may overcome this problem. This study therefore total genomic DNA from the parasite was used to construct