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Item Open Access Development of in-vitro methods to detect neutralizing antibody against the light chain of botulinum toxin in secondary botulinum toxin treatment failure patients(Mahidol University Library and Knowledge Center, 2021) Ishwor Sharma; Yuttana Srinoulprasert; Duangjit Kanistanon; Rungsima Wanitphakdeedechahad never undergone BoNT/A treatment. The BoNT/A tolerant group consisted of four stable individuals who had undergone BoNT/A treatment but were unable to respond. Only the patients with frontalis test positive were recruited into this study. The level...: This study would help us establish a non-cellular in-vitro immunological technique for detecting NAbs against the light chain of BoNT/A in STF patients in routine clinical practice and providing a rapid diagnosis. Furthermore, it has the potential