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Item Metadata only Isolation of genomic DNA fragments from jasmine rice which are differentially expresessed in various tissues(Mahidol University. Mahidol University Library and Knowledge Center, 1991) Kalaya Silawisas; Burachai Sonthayanon; Sakol PanyimA genomic DNA library of Oryza sativa subspecies indica cv. KDML 105 has been constructed by using bacteriophage lambda Gem-11 as a cloning vector. DNA fragments from the rice genome were obtained by partial Sau3A I digestion followed by size... probability of having cloned any DNA piece with an average size 18 kb in the library was about 0.95. About thirteen thousand plaques in this amplified library have been screened for clones which seems to be differentially expressed in sheath and leaf tissuesItem Metadata only Development of DNA probe for the RFLP analysis of DNA from Macrobrachium rosenbergii (de Man)(Mahidol University. Mahidol University Library and Knowledge Center, 2024) Supavadee Prakongsai; Thanit Kusamran; Worachart Sirawaraporn; Boonserm Poolsanguan) about 86 degree C corresponding to 43% G-C content. The genomic library of M.rosenbergii was constructed from Sau3AI-digested hepatopancreas DNA of a male prawn and ligated to BamHI site of pUC18. The transformants were selected through ampicillinItem Metadata only Molecular genetic characterization and expression studies of sucrose transporter genes in cassava (manihot exculenta crantz)(Mahidol University. Mahidol University Library and Knowledge Center, 2006) Apaporn Rattanakitti; Jarunya Narangajavana. This research aims to identify cDNAs encoding SUTs and investigate their gene expression patterns in cassava. We took an initial step by screening both storage root and leaf cDNA libraries for cDNA encoding SUT type 1, 2 and 4. A MeSUT2 cDNA was identified... in the leaf cDNA library, whereas MeSUTI, MeSUT4-1 and MeSUT4-2 cDNAs were isolated from storage root cDNA library. The translated amino acid sequences of MeSUTI, MeSUT2, MeSUT4-1 and MeSUT4-2 showed the highest similarity to Ricinus communis ReScr1 (82Item Metadata only Some approaches for the molecular cloning of Plasmodium vivax DNA in Escherichia coli(Mahidol University. Mahidol University Library and Knowledge Center, 2024) Manee Pookanjanatavip; Prapon Wilairat; Sakol Panyim; Yongyuth Yuthavong.13% respectively. Passage of the blood samples through a CF-11 cellulose column failed to remove white cells, and only resulted in loss of half of packed red cell volume. Three approaches were taken for the construction of P. vivax DNA library. Firstly, the less... that were obtained from all libraries were first screened by colony hybridization, and potential P. vivax specific clones were then selected. The specificity of these clones were confirmed by Southern or dot-blot hybridization. However, despite a great dealItem Metadata only Proteomic analysis of differently expressed proteins in penaeus vannamei survivors after acute yellow head virus infection(Mahidol University. Mahidol University Library and Knowledge Center, 2023) Triwit Rattanarojpong; Skorn Mongkolsuk; Fkegekm, Timothy W.promoter from fosmid DNA library of P. monodon, some clones from the library possibly contained SCP promoter. The results presented here provide preliminary data on the interaction between YHV and more insights into the pathogenesis and mechanism of YHVItem Metadata only Molecular cloning of glucoamylase gene from cephalosporium eichhorniae(Mahidol University. Mahidol University Library and Knowledge Center, 2023) Manee Leartvibhas; Chuanpit De-Eknamkul; Chuenchit Boonchird; Flegel, Timothy W.The Cephalosporium eichhorniae glucoamylase-encoding gene (3.1 kb EcoRI fragment) was isolated from ʎgt10 genomic library using non radioisotope labelled (enhanced chemiluminescence; ECL) synthetic oligonucleotide oliBT2 as a probe. The sequence...ยีนกลูโคอะไมเลสขนาด 3.1 kb ที่มีปลาย EcoRl ถูกคัดแยกออกมาจาก library ของเชื้อ Cephalosporium eichhorniae ที่สร้างขึ้นโดยใช้ ʎgt10 เป็นดีเอ็นเอ พาหะและใช้ดีเอ็นเอติดตาม (oliBT2) ที่ติดฉลากด้วยสาร ปลอดรังสี (ECL-enhance chemiluminescence) ลำดับเบส ของItem Metadata only Construction of specific DNA probes for Opisthorchis viverrini detection and an attempt to clone genes coding for diagnostic epitopes(Mahidol University. Mahidol University Library and Knowledge Center, 2023) Rasana Sermswan; Stitaya Sirisinha; Sakol Panyim; Skorn Mongkolsuklibraries in pUC 8,9 and 12 plasmid and a bacteriophage expression vector Xgt11. Screening of these libraries with a number of different anti O.viverrini antisera were however unsuccessful. This is probably due to the interference of introns in the antigenicItem Metadata only A GPU implementation of 2nd acoustic least-squares reverse time migration(Mahidol University. Mahidol University Library and Knowledge Center, 2024) Phudit Sombutsirinun; Chaiwoot Boonyasiriwat; Sutthipong Noisagoolto minimize the objective function. Both forward- and backward-propagation of wavefields using the finite-difference method were performed on a single GPU using the NVIDIA CUDA library. For verification purposes, the GPU program of LSRTM was applied to aItem Metadata only Characterization of ribosomal DNA clones from rice(Mahidol University. Mahidol University Library and Knowledge Center, 2024) Pongsopee Attasart; Burachai Sonthayanon; Sakol PanyimA 3,699 base pairs long nucleotide sequence of an indica rice (KDML105) DNA clone, called GX3.6, was determined. Five additional DNA clones, designated as C13, C14, C32, C35 and C76, were also isolated from a KDML105 rice cDNA library and DNAItem Metadata only Oxidative stress response in Xanthomonas sp.(Mahidol University. Mahidol University Library and Knowledge Center, 2024) Sangpen Chamnongpol; Skorn Mongkolsuk; Burachai Sonthayanon; Watanalai Panbangredputative gene for alkyl hydroperoxide reductase (ahpX) was also isolated from a X.c.pv phaseoli DNA library by complementation of a ahp mutant of E.coli. The gene confered a high level resistance to both cumene and tert-butyl hydroperoxides in E