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Item Metadata only Detection of Vibrio parahaemolyticus hemolysin genes in frozen shrimps using multiplex polymerase chain reaction(Mahidol University. Mahidol University Library and Knowledge Center, 2005) Darunee Jaisawang; Orasa Suthienkul; Kanokrat Siripanichgon; Manee Chanamaof the microbiological criteria including aerobic total viable count, V. parahaemolyticus, coliforms and Escherichia coli. The lowest genomic DNA of three genes (ldh, tdh and trh) of V. parahaemolyticus type strain VP156-9-7-1993 was detected at 1 ng/μl by multiplex PCR.... Examining 19 strains of Vibrio spp. and enteric bacteria with these 3 genes by multiplex PCR resulted in 100% specificity. In 14% (14/100) of frozen shrimp enrichment samples V. parahaemolyticus was detected by both the conventional method and multiplex PCR