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Item Metadata only Perinatal transmission of HIV-1 subtype E : diagnosis and nucleotide sequence analysis of pol gene(Mahidol University. Mahidol University Library and Knowledge Center, 2000) Daoroong Piyasatjaboon; Ruengpung Sutthent; Kulkunya Chokephaibulkit; Prasert Auewarakul; Uraiwan KositanontItem Metadata only Studies of non-tuberculous mycobacteria in AIDS patients : 16S rRNA gene sequence, phenotype and drug susceptibility(Mahidol University. Mahidol University Library and Knowledge Center, 2003) Naiyanawan Prasong; Suporn FoongladdaItem Metadata only Nucleotide sequence analysis and strain improvement for high expression of penicillin G acylase gene from Bacillus megaterium UN-1(Mahidol University. Mahidol University Library and Knowledge Center, 2024) Kanchana Weeradechapon; Watanalai Panbangred; Vithaya Meevootisom; Chuenchit Boonchirdmegaterium UN-1, a high penicillin G acylase producer mutant of Bacillus megaterium ATCC 14945. Complete nucleotide sequence of pac gene was determined and found that the gene contained an open reading frame encoding for 802 amino acids with MW of about 92...,000 Da. This polypeptide consisted of a 26 amino acid signal peptide. The putative -35 and -10 sequences were TTGAAT and TATAAG, respectively. Comparison of pac nucleotide sequence to that from Arthrobacter viscosus revealed 97% homology. Results from SDSItem Metadata only Analysis of the nucleocapsid gene of hepatitis C virus isolated in Thailand(Mahidol University. Mahidol University Library and Knowledge Center, 2024) Rojana Kunkitti; Sirirurg Songsivilai; Tararaj Dharakul; Sunee KorbsrisateItem Metadata only Cloning and sequencing of hepatitis B virus genome adr subtype isolated in Thailand(Mahidol University. Mahidol University Library and Knowledge Center, 2024) Patama Monkongdee; Somsak Pantuwatana; Chuenchit Boonchird; Watanalai Panbangrednucleotide sequence was determined. The 3,215 bp sequences show the presence of 4 open reading frames (ORF) for PreS+HBsAg (174+226 amino acids), P (843 amino acids), HBcAg (212 amino acids) and X (154 amino acids). The gene locus organization... was not different from those of the other adr clones so far reported. It was found that all of the direct repeat of the undecanucleotide sequence (DR1) near the 5 ends of the short (S) and long (L) strands of HBV DNA and the two small direct repeat sequences (DR2Item Metadata only Cloning and characterization of the alkaline protease gene (alpA) from Aspergillus oryzae(Mahidol University. Mahidol University Library and Knowledge Center, 2023) Supaporn Cheevadhanarak; Flegel, Timothy W.; Amaret Bhumiratana; Skorn Mongkolsuk; Holt, Geoffreyprobes based on the published cDNA sequence [Tatsumi et al., Agric. Biol. Chem. 52(1988) 1887-1888]. The entire nucleated sequence of the genomic clone obtained was determined. By comparison with the published cDNA sequence, it was found that Alp... was encoded by four exons of 314, 445, 89, and 351 bp. Three introns, which interrupted the coding sequence, were 50, 59, and 56 bp in length. The gene contains a typical TATA box 103 base pairs upstream, from the ATG start codon, and a consensusItem Metadata only Preliminary study of genetic diversity of Pneumocystis Carinii F. SP. Hominis in Thailand(Mahidol University. Mahidol University Library and Knowledge Center, 2005) Suradej Siripattanapipong; Peerapan Tan-ariya; Mathirut Mungthin; Jeerapun Worapongin Thai patients is not available. The objective of this study is to identify the genotypes of this organism in HIV-infected Thai patients by sequence analysis of internal transcribed spacers (ITSs) regions 1 and 2. The bronchoalveolar lavage (BAL...) and sputum specimens from 28 HIV-infected Thai patients with PCP were used to analyze the genotypes. The criteria of type classification were based on a typing score described by Lee et al. and a scoring system that counts the frequency of novel alleles foundItem Metadata only HIV genetic variation in antiretroviral drug treated Thais from 2009-2011(Mahidol University. Mahidol University Library and Knowledge Center, 2024) Nattapol Narong; Ruengpung Sutthent; Wannee Kantakamalakul; Navin Horthongkhamand highly divergent groups based primarily on the analysis of genetic sequences coding for the envelope (env) and other structural (gag, pol) proteins, including M , O , N and P. The variations of envelope proteins of HIV-1 group M and HIV-1 group O are 30... are the major subtypes circulating in Thailand. This study aimed to analyze the genetic sequences of HIV from 675 collected clinical samples for detecting drug resistance genotype of HIV using nucleotide sequencing. Thirty-five of the 675 random samplesItem Metadata only Construction of specific DNA probes for Opisthorchis viverrini detection and an attempt to clone genes coding for diagnostic epitopes(Mahidol University. Mahidol University Library and Knowledge Center, 2023) Rasana Sermswan; Stitaya Sirisinha; Sakol Panyim; Skorn Mongkolsukis to develop specific DNA probes for detecting parasite eggs in stool samples. Two recombinant plasmids were selected and named pOV-P5 and pOV-A6. The pOV-P5 has a 1.4 Kb insert which cross hybridized with a 334 base pair (bp) insert fragment of pOV-A6. When... using as probes to hybridize with digested genomic DNA from O.viverrini, both plasmids gave similar tandemly repeated patterns. Sequence analysis of pOV-A6 suggests it to be the smallest unit of the repeated elements. This repeated unit was found