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Item Open Access Development of quantitative polymerase chain reaction assay for detection of scale drop disease virus (SDDV) in Asian sea bass(Mahidol University, 2021) Sukhontip Sriisan; Chuenchit Boonchird; Siripong Thitamadee; Saengchan Senapineither ATPase or MCP primers showed no cross amplification with DNA extracted from 12 viruses and bacteria commonly found in aquatic animals. However, the former protocol had a detection limit of 2 viral copies per reaction while the latter had 20 copies