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Subject: Immunology and Microbiology

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Now showing 1 - 10 of 465
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    Evaluation for the Clinical Diagnosis of Pythium insidiosum Using a Single-Tube Nested PCR
    (2013-08-16) Yordhathai Thongsri; Lumyai Wonglakorn; Angkana Chaiprasert; Lucie Svobodova; Petr Hamal; Maitree Pakarasang; Chularut Prariyachatigul; Khon Kaen University; Mahidol University; University Hospital Olomouc
    Pythiosis is a rare infectious disease caused by Pythium insidiosum, which typically occurs in tropical and subtropical regions. The high mortality rate may be in consequence of the lack of diagnosis. The objective of this study was to evaluate... reliability of a new single-tube nested PCR for detection of P. insidiosum DNA. A total of 78 clinical isolates of various fungi and bacteria, 106 clinical specimens and 80 simulated positive blood samples were tested. The developed primer pairs CPL6-CPR8
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    Prospective evaluation of a rapid immunochromogenic cassette test for the diagnosis of melioidosis in northeast Thailand
    (2006-01-01) Allen C. Cheng; Sharon J. Peacock; Direk Limmathurotsakul; Gumphol Wongsuvan; Wirongrong Chierakul; Premjit Amornchai; Nongluk Getchalarat; Wipada Chaowagul; Nicholas J. White; Nicholas P.J. Day; Vanaporn Wuthiekanun; Mahidol University; Menzies School of Health Research; Sappasitthiprasong Hospital; Nuffield Department of Clinical Medicine; Geelong Hospital
    A prospective study was performed to compare a rapid immunochromogenic cassette test (ICT) with the indirect haemagglutination assay (IHA) and clinical rules for the diagnosis of melioidosis in an endemic area. The sensitivity and specificity... of the IgG ICT was 86% and 47%, and the IgM ICT was 82% and 47%, respectively. These were similar to the results for IHA (sensitivity 73%, specificity 64%) and clinical rules (73% and 37%). ICT lacks clinical utility as a result of high background rates
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    Prospective clinical evaluation of the accuracy of 16S rRNA real-time PCR assay for the diagnosis of melioidosis
    (2007-11-01) Narisara Chantratita; Vanaporn Wuthiekanun; Direk Limmathurotsakul; Aunchalee Thanwisai; Wasun Chantratita; N. P J Day; Sharon J. Peacock; Mahidol University
    The accuracy of a Burkholderia pseudomallei 16s rRNA real-time PCR assay was evaluated against culture for the diagnosis of melioidosis in Thailand. A total of 846 samples were obtained from 383 patients with suspected melioidosis. One or more... patients with positive blood cultures was 2.4 CFU/ml (0.2-13.5 CFU/ml); this may explain the low sensitivity of PCR for this specimen. The PCR assay described here is not sufficiently sensitive to replace culture in our clinical setting. Copyright © 2007
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    African green monkeys recapitulate the clinical experience with replication of live attenuated pandemic influenza virus vaccine candidates
    (2014-01-01) Yumiko Matsuoka; Amorsolo Suguitan; Marlene Orandle; Myeisha Paskel; Kobporn Boonnak; Donald J. Gardner; Friederike Feldmann; Heinz Feldmann; Michael Marino; Hong Jin; George Kemble; Kanta Subbarao; National Institute of Allergy and Infectious Diseases; National Institutes of Health, Bethesda; MedImmune, Inc.; American University of the Caribbean; Mahidol University; FDA Center for Biologics Evaluation and Research; 3-V Biosciences, Inc.
    with homologous and heterologous wild-type (wt) viruses of the corresponding subtypes. However, when these vaccine candidates were evaluated in phase I clinical trials, there were inconsistencies between the observations in animal models and in humans. The vaccine... in humans and evaluated African green monkeys (AGMs) as a nonhuman primate model. The distribution of sialic acid (SA) receptors in the respiratory tract of AGMs was similar to that in humans. We evaluated the replication of wt and ca viruses of avian
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    Perioperative immediate hypersensitivity incidence, clinical characteristics, and outcomes after allergological evaluation: A multi-disciplinary protocol from tertiary hospital, Thailand
    (2025-09-01) Krikeerati T.; Wongsa C.; Thongngarm T.; Rujirawan P.; Borrisut N.; Wongsripuemtet P.; Tuchinda P.; Srinoulprasert Y.; Subchookul C.; Veerapong C.; Khunsakdeeyodom P.; Sompornrattanaphan M.; Krikeerati T.; Mahidol University
    Background: Perioperative immediate hypersensitivity reaction (POH) is an immediate hypersensitivity reaction during an anesthesiologist monitored procedure. We report data of clinically-suspected POH (csPOH) patients undergoing an allergist... with csPOH was conducted at Siriraj Hospital, Thailand, covering events from January 2018 to August 2022. Diagnostic workup for POH by the allergist included an initial assessment, followed by comprehensive allergological evaluation. Patients were
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    Preliminary clinical evaluation of a protein chip for tumor marker serodiagnosis of various cancers
    (2003-09-01) Kruavon Balachandra; Pikul Laisupasin; Panadda Dhepakson; Jiranan Warachit; Udom Jantraraksri; Surapol Issaragrisil; Xiang Li Yang; Geng Xi Hu; Mahidol University; Institute of Biochemistry and Cell Biology, Shanghai Institute for Biological Sciences Chinese Academy of Sciences
    This preliminary study aimed to investigate sensitivity and specificity of a protein chip system for multi-tumor marker serodiagnosis of ten types of cancers, and to understand the possible clinical applications of this protein chip for the Thai... population. The specific cancers diagnosed by this protein chip are lung, breast, liver, cervix, colo-rectal, stomach, ovary, esophagus, prostate and pancreas cancers. We analyzed 215 serum samples of which 165 were obtained from clinically confirmed cancer
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    Diagnostic Performances of an in-House Immunochromatography Test Based on the Monoclonal Antibody 18B7 to Glucuronoxylomannan for Clinical Suspected Cryptococcosis: a Large-Scale Prototype Evaluation in Northern Thailand
    (2024-10-01) Pruksaphon K.; Amsri A.; Thammasit P.; Nosanchuk J.D.; Aiumurai P.; Youngchim S.; Pruksaphon K.; Mahidol University
    were 99.10%, 97.61%, and 97.83%, respectively. The agreement kappa (κ) coefficient was 0.968 based on the retrospective evaluation of 580 specimens from patients living in northern Thailand with clinically suspected cryptococcosis. Conclusion: The data
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    Evaluation of intravenous fosfomycin disodium dosing regimens in critically ill patients for treatment of carbapenem-resistant enterobacterales infections using monte carlo simulation
    (2020-09-01) Pannee Leelawattanachai; Thitima Wattanavijitkul; Taniya Paiboonvong; Rongpong Plongla; Tanittha Chatsuwan; Sang Usayaporn; Wichit Nosoongnoen; Preecha Montakantikul; Chulalongkorn University; Rangsit University; King Chulalongkorn Memorial Hospital, Faculty of Medicine Chulalongkorn University; Vajira Hospital; Mahidol University
    ) for evaluation of IVFOS dosing regimens in critically ill patients with CRE infections. The dosing regimens in critically ill patients with various creatinine clearance were evaluated with MCS using minimum inhibitory concentration (MIC) distributions... of fosfomycin against CRE clinical isolates in Thailand and the 24 h area under the plasma drug concentration–time curve over the minimum inhibitory concentration (AUC0-24/MIC) of ≥21.5 to be a target for IVFOS. The achieved goal of the probability of target
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    Triangular test design to evaluate tinidazole in the prevention of Plasmodium vivax relapse
    (2013-06-03) Louis Macareo; Khin Maung Lwin; Phaik Yeong Cheah; Prayoon Yuentrakul; R. Scott Miller; Francois Nosten; Walter Reed Army Institute of Research; Shoklo Malaria Research Unit; Mahidol University; Nuffield Department of Clinical Medicine
    and economic advantages of this study design to evaluate similarly situated drugs. Trial registration. ClinicalTrials.gov NCT00811096. © 2013 Macareo et al.; licensee BioMed Central Ltd.... in man. Presented here are the results of a pilot phase II, randomized, open-label study conducted along the Thai-Myanmar border designed to evaluate the efficacy of tinidazole to prevent relapse of P. vivax when administered with chloroquine. Methods
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    Clinical evaluation of a newly developed automated massively parallel sequencing assay for hepatitis C virus genotyping and detection of resistance-association variants. Comparison with a line probe assay
    (2017-11-01) Narathon Manee; Nipa Thongbaiphet; Ekawat Pasomsub; Wasun Chantratita; Mahidol University
    evaluation of exact HCV genotyping in a newly developed automated-massively parallel sequencing (MPS) system, versus the established Line probe assay 2.0 (LiPA), substantiated by Sanger sequencing, using 120 previously identified-HCV RNA positive specimens