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Subject: Immunology and Microbiology

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Now showing 1 - 10 of 37
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    FlexiChip package: An universal microarray with a dedicated analysis software for high-thoughput SNPs detection linked to anti-malarial drug resistance
    (2009-11-30) Nicolas Steenkeste; Marie Agnès Dillies; Nimol Khim; Odile Sismeiro; Sophy Chy; Pharath Lim; Andreas Crameri; Christiane Bouchier; Odile Mercereau-Puijalon; Hans Peter Beck; Mallika Imwong; Arjen M. Dondorp; Duong Socheat; Christophe Rogier; Jean Yves Coppée; Frédéric Ariey; Institut Pasteur du Cambodge; Institut Pasteur, Paris; Amunix, Inc.; GENOPOLE; Swiss Tropical and Public Health Institute (Swiss TPH); Mahidol University; Centre for Clinical Vaccinology and Tropical Medicine; National Center for Parasitology, Entomology and Malaria Control; IMTSSA Institut de Medecine Tropicale du Service de Sante des Armees
    of practical methods enabling high throughput analysis. Here a new Zip-code array is described, called FlexiChip, linked to a dedicated software program, which largely overcomes this problem. Methods. Previously published microarray probes detecting single...-nucleotide polymorphisms (SNP) associated with parasite resistance to anti-malarial drugs (ResMalChip) were adapted for a universal microarray FlexiChip format. To evaluate the overall sensitivity of the FlexiChip package (microarray + software), the results
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    Microarrayed Allergen Molecules Distinguish IgE Sensitisation to Blomia tropicalis and Dermatophagoides pteronyssinus
    (2026-01-01) Dsouza N.; Phanthong S.; Huang H.J.; Weber M.; Sarzsinszky E.; Zieglmayer P.; Pauli G.; Focke-Tejkl M.; Tulaev M.; Todorovic N.; Keller W.; Schlederer T.; Caraballo L.; Tungtrongchitr A.; Valenta R.; Tantilipikorn P.; Vrtala S.; Dsouza N.; Mahidol University
    . There is only limited IgE cross-reactivity between Dermatophagoides and Blomia. Objective: To produce a chip containing purified microarrayed Blomia tropicalis (Blo t) and Dermatophagoides pteronyssinus allergens (Der p) capable of identifying patients with a... genuine Blo t or Der p IgE sensitisation, co-sensitisation and/or cross-sensitisation. Methods: Chips containing seven purified Blo t and thirteen Der p allergens were generated by microarray technology and tested for IgE and IgG reactivity in HDM
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    Microarray identification of Clostridium difficile core components and divergent regions associated with host origin
    (2009-06-01) Tavan Janvilisri; Joy Scaria; Angela D. Thompson; Ainsley Nicholson; Brandi M. Limbago; Luis G. Arroyo; J. Glenn Songer; Yrjö T. Gröhn; Yung Fu Chang; Cornell University; National Center for Emerging and Zoonotic Infectious Diseases; Ontario Veterinary College; University of Arizona; Mahidol University
    evaluated the genomic content of 73 C. difficile strains isolated from humans, horses, cattle, and pigs by comparative genomic hybridization with microarrays containing coding sequences from C. difficile strains 630 and QCD-32g58. The sequenced genome of C
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    Microarrays reveal that each of the ten dominant lineages of Staphylococcus aureus has a unique combination of surface-associated and regulatory genes
    (2006-01-01) Jodi A. Lindsay; Catrin E. Moore; Nicholas P. Day; Sharon J. Peacock; Adam A. Witney; Richard A. Stabler; Sarah E. Husain; Philip D. Butcher; Jason Hinds; St George's University of London; University of Oxford; Mahidol University; London School of Hygiene & Tropical Medicine; Imperial College London
    carriage isolates from healthy donors using a microarray spotted with PCR products representing every gene from the seven S. aureus sequencing projects. The core genes common to all strains were identified, and 10 dominant lineages of S. aureus were clearly
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    Leptospire genomic diversity revealed by microarray-based comparative genomic hybridization
    (2012-05-01) Broderick Eribo; Sirima Mingmongkolchai; Tingfen Yan; Padunsri Dubbs; Karen E. Nelson; Howard University; Mahidol University; J. Craig Venter Institute
    Comparative genomic hybridization was used to compare genetic diversity of five strains of Leptospira (Leptospira interrogans serovars Bratislava, Canicola, and Hebdomadis and Leptospira kirschneri serovars Cynopteri and Grippotyphosa). The array was designed based on two available sequenced Leptospira reference genomes, those of L. interrogans serovar Copenhageni and L. interrogans serovar Lai. A comparison of genetic contents showed that L. interrogans serovar Bratislava was closest to the reference genomes while L. kirschneri serovar Grippotyphosa had the least similarity to the reference genomes. Cluster analysis indicated that L. interrogans serovars Bratislava and Hebdomadis clustered together first, followed by L. interrogans serovar Canicola, before the two L. kirschneri strains. Confirmed/potential virulence factors identified in previous research were also detected in the tested strains. © 2012, American Society for Microbiology.
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    Differential gene expression profiles of human monocyte-derived antigen presenting cells in response to Penicillium marneffei: Roles of DC-SIGN (CD209) in fungal cell uptake
    (2008-12-01) Piyaporn Ngaosuwankul; Piyapong Pongtanalert; Aneke Engering; Sansanee C. Chaiyaroj; Mahidol University; Armed Forces Research Institute of Medical Sciences, Thailand; Chulabhorn Research Institute
    DNA microarray technology was used to determine the gene expression profile of human monocyte-derived macrophages (hMDMs) after stimulation by Penicillium marneffei yeast. The expression levels of 175 macrophage genes were found to be altered by a... upregulated in hMDMs. Functional analysis of the adhesion of P. marneffei to dendritic cell-specific intercellular adhesion molecule-3 grabbing non-integrin (DC-SIGN, CD209) was performed in hMoDCs since the microarray data revealed an increased expression
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    A genetically hard-wired metabolic transcriptome in Plasmodium falciparum fails to mount protective responses to lethal antifolates
    (2008-11-01) Karthikeyan Ganesan; Napawan Ponmee; Lei Jiang; Joseph W. Fowble; John White; Sumalee Kamchonwongpaisan; Yongyuth Yuthavong; Prapon Wilairat; Pradipsinh K. Rathod; University of Washington, Seattle; Mahidol University; Thailand National Center for Genetic Engineering and Biotechnology; Orchid Research Laboratories Limited
    Genome sequences of Plasmodium falciparum allow for global analysis of drug responses to antimalarial agents. It was of interest to learn how DNA microarrays may be used to study drug action in malaria parasites. In one large, tightly controlled... study involving 123 microarray hybridizations between cDNA from isogenic drug-sensitive and drug-resistant parasites, a lethal antifolate (WR99210) failed to over-produce RNA for the genetically proven principal target, dihydrofolate reductasethymidylate
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    Spinal neuronal excitability and neuroinflammation in a model of chemotherapeutic neuropathic pain: targeting the resolution pathways
    (2020-12-01) Pongsatorn Meesawatsom; Gareth Hathway; Andrew Bennett; Dumitru Constantin-Teodosiu; Victoria Chapman; University of Nottingham; Mahidol University
    ). Behavioural pain responses were measured, and either single unit electrophysiological recordings of dorsal horn wide dynamic range (WDR) neurones were performed, or mRNA microarray analysis of the dorsal horn of the spinal cord was undertaken. Results: PCX... cold and exhibiting spontaneous activity) compared to control (p < 0.05). Microarray analysis revealed changes in proinflammatory pathways (Tlr, Tnfrsf1a, Nlrp1a, Cxcr1, Cxcr5, Ccr1, Cx3cr1) and anti-inflammatory lipid resolvin pathways (Alox5ap, Cyp2j4
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    Global gene expression profiling of Plasmodium falciparum in response to the anti-malarial drug pyronaridine
    (2011-08-22) Kanyanan Kritsiriwuthinan; Philip J. Shaw; Chayaphat Wongsombat; Porntip Chavalitshewinkoon-Petmitr; Sumalee Kamchonwongpaisan; Thailand National Center for Genetic Engineering and Biotechnology; Mahidol University
    , and parasites were harvested from culture after 4 and 24 hours exposure. Global transcriptional changes effected by drug treatment were investigated using DNA microarrays. Results: After a 4 h drug exposure, PN induced a greater degree of transcriptional.... However, PN appears to exert a more rapid response than CQ. The faster action of PN may explain why PN is more efficacious than CQ, particularly against CQ resistant isolates. In agreement with several other microarray studies of drug action
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    Biomarker selection and classification of "- Omics " data using a two-step bayes classification framework
    (2013-10-07) Anunchai Assawamakin; Supakit Prueksaaroon; Supasak Kulawonganunchai; Philip James Shaw; Vara Varavithya; Taneth Ruangrajitpakorn; Sissades Tongsima; Mahidol University; Thammasat University; Thailand National Center for Genetic Engineering and Biotechnology; King Mongkut's University of Technology North Bangkok; Natioanl Electronic and Computer Technology Center
    types of -omics datasets including gene expression microarray, single nucleotide polymorphism microarray (SNParray), and surface-enhanced laser desorption/ionization time-of-flight (SELDI-TOF) proteomic data. The proposed two-step Bayes classification