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Subject: Medicine

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    Is sabin-feldman dye test using T. Gondii tachyzoites from animal inoculation still the best method for detecting toxoplasma gondii antibodies?
    (2010-09-01) Ruenruetai Udonsom; Ruangrat Buddhirongawatr; Yaowalark Sukthana; Mahidol University
    Although the Sabin-Feldman dye test is the gold standard for detecting Toxoplasma antibodies in human, it is performed only in reference laboratories because live virulent T. gondii are used for the test. We collected 210 human serum samples... and tested them by the dye test using in vivo tachyzoites (conventional method) then compared these results with three other methods: a dye test using cell culture-derived T. gondii tachyzoites and indirect immunofluorescent antibody tests (IFAT) using
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    Comparison of indirect immunofluorescent antibody test and Sabin-Feldman dye test for detection of Toxoplasma gondii antibody in Thai pregnant women
    (2004-12-01) Uraiwan Krainara; Supatra Thongrungkiat; Wipawee Usawattanakul; Porntip Petmitr; Yaowalak Sukthana; Department of Protozoology; Mahidol University
    The aim of this study is to determine the specific IgG antibody for Toxoplasma gondii in pregnant Thai women by IFAT, using formalin-fixed whole tachyzoites and comparing the sensitivity and specificity with those of the Sabin-Feldman dye test (SFDT... and 95.9% specificity, when compared with Sabin-Feldman dye test. IFAT is not time-consuming, easy to perform, and formalin-fixed whole antigens can be kept for a long time. IFAT, therefore, is recommended as a selective test, which can be used
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    Predictive value of latex agglutination test in serological screening for Toxoplasma gondii
    (2001-06-01) Yaowalark Sukthana; Thaiyooth Chintana; Waraporn Supatanapong; Chutatip Siripan; Amorn Lekkla; Rachatawan Cheabchalrad; Mahidol University
    -Feldman dye test. Five hundred serum samples were collected from 200 blood donors and 100 each from pregnant women, kidney recipients and HIV infected persons. Eighty (16.0 %) out of 500 subjects were positive for Toxoplasma IgG antibody by ToxoScreen DA... (bioMerieux) compared with 57(11.4%) by Sabin-Feldman dye test. The sensivity and specificity of Toxo-Screen DA (bioMerieux) were 100 % and 94.8 % respectively which were similar to previous reports from the area of high prevalence of Toxoplasma
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    Contact allergy to hair cosmetic allergens in Thailand
    (2019-12-01) Waranya Boonchai; Waranaree Winayanuwattikun; Pichaya Limphoka; Thanisorn Sukakul; Faculty of Medicine, Siriraj Hospital, Mahidol University
    patch tested. Of the hair cosmetic allergen categories, preservatives had the highest rate of positive reactions, followed by surfactants and hair dyes. Perming agents were less problematic in comparison. The hands, head, and neck were the predominately...© 2019 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd Background: Hair cosmetics such as hair dyes, bleaching, waving, and cleansing products are composed of numerous chemical ingredients. Allergic reactions to these contact allergens
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    The permeability of the dentine and other tissues that are exposed at the tip of a rat incisor
    (2000-01-01) K. Vongsavan; N. Vongsavan; B. Matthews; Mahidol University; University of Bristol, Faculty of Medicine and Dentistry
    Evans blue dye. The incisal surface was prepared by etching it with acid or removing 1 mm by fracturing or with a diamond disc followed by etching. In some cases, 1.5-3.0 mm was removed to expose the soft tissue of the pulp. The dye was applied for 30... min, then longitudinal sections of the crown were cut and examined microscopically to determine the extent to which the dye had diffused into the underlying tissues. In only those teeth in which the dye had been applied direct to the pulpal soft tissue
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    Antiseptics for preventing omphalitis
    (2002-02-01) Sopapan Panyavudhikrai; Somwang Danchaivjitr; Chanchai Vantanasiri; Suwanna Trakulsomboon; Thrathip Kolatat; Chertsak Dhiraputra; Varaporn Poomsuwan; Nitaya Srirapol; Mahidol University
    (Triple dye) or group B (70% Alcohol). The infant with omphalitis was assessed by a pediatrician or a neonatology fellow. At home, the same antiseptic will be continually applied to the umbilical stump daily until a few days after cord detachment. Relative... risk was calculated and statistical significance was tested by Chi-square test. Results: Four hundred and twenty-seven infants were enrolled. Birth weight, gestational age and gender of the infants in both groups were not different. There were no known
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    Detection of Toxolasma gondii in captive wild felids.
    (2006-12-01) Ruangrat Buddhirongawatr; Siriporn Tungsudjai; Kridsada Chaichoune; Charoonluk Sangloung; Nitipan Tantawiwattananon; Rassameepen Phonaknguen; Yaowalark Sukthana; Mahidol University
    Toxoplasma gondii can infect all species of warm-blooded animals, including humans, and causes serious diseases in immunocompromized hosts. Live tachyzoites derived from serial passage in HeLa culture were used in the Sabin-Feldman dye test... can be used successfully as a source of live organisms in a gold standard Sabin-Feldman dye test, which is simpler, cheaper and less ethically sensitive than in vivo inoculation.
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    Live imaging of the genetically intractable obligate intracellular bacteria Orientia tsutsugamushi using a panel of fluorescent dyes
    (2016-11-01) Sharanjeet Atwal; Suparat Giengkam; Michael VanNieuwenhze; Jeanne Salje; Mahidol University; Nuffield Department of Clinical Medicine; Indiana University
    , which can be used to delineate the cytoplasm, nucleic acids, cell membrane or peptidoglycan of living bacterial cells. We have tested these in the context of the important but neglected human pathogen Orientia tsutsugamushi but expect
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    Sentinel lymph node biopsy after neoadjuvant treatment of breast cancer using blue dye, radioisotope, and indocyanine green: Prospective cohort study
    (2020-11-01) Prakasit Chirappapha; Tanet Chatmongkonwat; Panuwat Lertsithichai; Wiriya Pipatsakulroj; Chanika Sritara; Thongchai Sukarayothin; Faculty of Medicine, Ramathibodi Hospital, Mahidol University
    © 2020 The Authors Background: The breast cancer treatment paradigm has shifted to neoadjuvant treatment. There are many advantages to neoadjuvant treatment, such as tumor downsizing, in vivo tumor biology testing, treating micrometastasis....73 cm. The lymph node size was 1.8 ± 0.96 cm. The detection rates at the individual level were 95.23% with ICG, 85.71% with isosulfan blue, and 85.71% with a radioisotope. The detection rate increased up to 100% when the ICG and blue dye methods were
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    Microleakage of two fluoride-releasing sealants when applied following saliva contamination
    (2013-01-01) Praphasri Rirattanapong; Kadkao Vongsavan; Rudee Surarit; Mahidol University
    for 24 hours and sectioned in the bucco-lingual direction. Dye penetration (microleakage) was examined with a 25x polarized light microscope and measured by a computerized- calculated method. Data were compared with the Mann-Whitney U test at significance... as Clinpro® and Group B, a glass-ionomer sealant marketed as Fuji VII™. After sealant application, all the teeth were thermocycled for 2,000 cycles and coated with nail varnish 1.0 mm from the sealed areas. The teeth were stained with 2% methylene blue dye