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Publication Open Access Validity and reliability of quality of recovery-35 Thai version: a prospective questionnaire-based study(2016) Siriporn Pitimana-aree; Suthipol Udompanthurak; Saowaphak Lapmahapaisan; Matula Tareerath; Aungsumat Wangdee; Mahidol University. Faculty of Medicine Siriraj Hospital. Department of Anesthesiologyevaluation. The QoR-40, however, is English-based and was tested and validated in Caucasian patients, a population that is culturally and behaviorally different from the Thai population. The objective of this study was to translate and modify the original... English-language QoR-40 into the Thai language and evaluate the Quality of Recovery-35 Thai version for measuring health outcomes in Thai patients after surgery and anesthesia. Methods: Translation was performed according to internationally recognizedPublication Open Access ข้อควรคำนึงในการเป็นล่ามภาษามือวิชาชีพ(2548) Kenzel, Hannah; วิไลรัตน์ ศรีคำ; มหาวิทยาลัยมหิดล. วิทยาลัยราชสุดาPublication Open Access Proteomic analysis of Chikungunya virus infected microgial cells(2012-04) Bizunesh Abere; Nitwara Wikan; Sukathida Ubol; Prasert Auewarakul; Atchara Paemanee; Suthathip Kittisenachai; Sittiruk Roytrakul; Duncan R. Smith; Mahidol University. Institute of Molecular Biosciences. Molecular Pathology Laboratory; Mahidol University. Faculty of Science. Department of Microbiology; Mahidol University. Center for Emerging and Neglected Infectious Disease.in response to infection did not support a global inhibition of either normal or IRES-mediated translation, but was consistent with the targeting of specific cellular pathways including those regulating innate antiviral mechanisms.Publication Open Access Phosphoproteomic analysis of apoptotic hematopoietic stem cells from hemoglobin E/b-thalassemia(2011) Saranyoo Ponnikorn; Tasanee Panichakul; Kitima Sresanga; Chokdee Wongborisuth; Sittiruk Roytrakul; Suradej Hongeng; Sumalee Tungpradabkul; Mahidol University. Faculty of Science. Department of BiochemistryBackground: Hemoglobin E/b-thalassemia is particularly common in Southeast Asia and has variable symptoms ranging from mild to severe anemia. Previous investigations demonstrated the remarkable symptoms of bthalassemia in terms of the acceleration of apoptotic cell death. Ineffective erythropoiesis has been studied in human hematopoietic stem cells, however the distinct apoptotic mechanism was unclear. Methods: The phosphoproteome of bone marrow HSCs/CD34+ cells from HbE/b-thalassemic patients was analyzed using IMAC phosphoprotein isolation followed by LC-MS/MS detection. Decyder MS software was used to quantitate differentially expressed proteins in 3 patients and 2 normal donors. The differentially expressed proteins from HSCs/CD34+ cells were compared with HbE/b-thalassemia and normal HSCs. Results: A significant change in abundance of 229 phosphoproteins was demonstrated. Importantly, the analysis of the candidate proteins revealed a high abundance of proteins that are commonly found in apoptotic cells including cytochrome C, caspase 6 and apoptosis inducing factors. Moreover, in the HSCs patients a significant increase was observed in a specific type of phosphoserine/threonine binding protein, which is known to act as an important signal mediator for the regulation of cell survival and apoptosis in HbE/b-thalassemia. Conclusions: Our study used a novel method to investigate proteins that influence a particular pathway in a given disease or physiological condition. Ultimately, phosphoproteome profiling in HbE/b-thalassemic stem cells is an effective method to further investigate the cell death mechanism of ineffective erythropoiesis in b-thalassemia. Our report provides a comprehensive phosphoproteome, an important resource for the study of ineffective erythropoiesis and developing therapies for HbE/b-thalassemia.Publication Open Access Customized-language voice survey on mobile devices for text and image data collection among ethnic groups in Thailand: a proof-of-concept study.(2014-03-06) Kasemsak Jandee; เกษมศักดิ์ จันดี; Saranath Lawpoolsri; สารนาถ ล้อพูลศรี; Pimsurang Taechaboonsermsak; พิมพ์สุรางค์ เตชะบุญเสริมศักดิ์; Amnat Khamsiriwatchara; Peerawat Wansatid; Jaranit Kaewkungwal; จรณิต แก้วกังวาล; Jaranit Kaewkungwal; Mahidol University. Faculty of Tropical Medicine. Center of Excellence for Biomedical and Public Health Informatics (BIOPHICS); Mahidol University. Faculty of Tropical Medicine. Department of Tropical Hygiene.; Mahidol University. Faculty of Public Health. Department of Family Health.questionnaires in Thai language were translated to each ethnic language by the interviewer/translator when interviewing the study participant. The customized-language voice-based questionnaires were programmed to a smartphone tablet in six, selectable dialect... and better approach than standard translated paper-based questionnaires for public health surveys, especially when collecting data among ethnic and hard-to-reach groups residing in multilanguage-speaking settings.Publication Open Access การแปลและการหาค่าความเชื่อมั่นของแบบสอบถามความสามารถในการเดินสำหรับผู้ป่วยโรคหลอดเลือดแดงส่วนปลายอุดตันฉบับภาษาไทย(2558) เกศศิริ วงษ์คงคํา; Kessiri Wongkongkam; มหาวิทยาลัยมหิดล. คณะพยาบาลศาสตร์are the limitation for these walking ability tests. Thailand has no report about the tools for assessment the patients’ walking ability. Therefore, this article was written to describe the translation and validation process of the Walking Impairment Questionnaire... (WIQ) from English version into Thai version by using Forward-and-backward translation method of Brislin. WIQ in Thai version has the Content Validity Index equal 1.00 and the Cronbach’s alpha coefficient was .96Publication Open Access Validity, reliability and interpretability of the Thai version of the urticaria control test (UCT)(2016) Kanokvalai Kulthanan; Leena Chularojanamontri; Papapit Tuchinda; Chuda Rujitharanawong; Maurer, Marcus; Weller, Karsten; Mahidol University. Faculty of Medicine Siriraj Hospital. Department of Dermatologyaimed to investigate the validity, reliability, screening accuracy, sensitivity to change and MCID of the linguistically validated translation of the UCT into the Thai language for assessing CU in the Thai population. Methods: A structured translationPublication Open Access Micro-plasticity of genomes as illustrated by the evolution of glutathione transferases in 12 Drosophila species(2014) Chonticha Saisawang; Ketterman, Albert J.; Mahidol University. Institute of Molecular BiosciencesGlutathione transferases (GST) are an ancient superfamily comprising a large number of paralogous proteins in a single organism. This multiplicity of GSTs has allowed the copies to diverge for neofunctionalization with proposed roles ranging from detoxication and oxidative stress response to involvement in signal transduction cascades. We performed a comparative genomic analysis using FlyBase annotations and Drosophila melanogaster GST sequences as templates to further annotate the GST orthologs in the 12 Drosophila sequenced genomes. We found that GST genes in the Drosophila subgenera have undergone repeated local duplications followed by transposition, inversion, and micro-rearrangements of these copies. The colinearity and orientations of the orthologous GST genes appear to be unique in many of the species which suggests that genomic rearrangement events have occurred multiple times during speciation. The high microplasticity of the genomes appears to have a functional contribution utilized for evolution of this gene family.Publication Open Access Comprehensive proteomic analysis of white blood cells from chikungunya fever patients of different severities(2014) Nitwara Wikan; Sarawut Khongwichit; Weerawat Phuklia; Sukathida Ubol; Tipparat Thonsakulprasert; Montri Thannagith; Duangrudee Tanramluk; Atchara Paemanee; Suthathip Kittisenachai; Sittiruk Roytrakul; Smith, Duncan R; Mahidol University. Institute of Molecular Biosciences; Mahidol University. Center for Emerging and Neglected Infectious DiseasesBackground: Chikungunya fever (CHIKF) is a recently re-emerged mosquito transmitted viral disease caused by the chikungunya virus (CHIKV), an Alphavirus belonging to the family Togaviridae. Infection of humans with CHIKV can result in CHIKF of variable severity, although the factors mediating disease severity remain poorly defined. Methods: White blood cells were isolated from blood samples collected during the 2009-2010 CHIKF outbreak in Thailand. Clinical presentation and viral load data were used to classify samples into three groups, namely non chikungunya fever (non-CHIKF), mild CHIKF, and severe CHIKF. Five samples from each group were analyzed for protein expression by GeLC-MS/MS. Results: CHIKV proteins (structural and non-structural) were found only in CHIKF samples. A total of 3505 human proteins were identified, with 68 proteins only present in non-CHIKF samples. A total of 240 proteins were found only in CHIKF samples, of which 65 and 46 were found only in mild and severe CHIKF samples respectively. Proteins with altered expression mapped predominantly to cellular signaling pathways (including toll-like receptor and PI3K-Akt signaling) although many other processes showed altered expression as a result of CHIKV infection. Expression of proteins consistent with the activation of the inflammasome was detected, and quantitation of (pro)-caspase 1 at the protein and RNA levels showed an association with disease severity. Conclusions: This study confirms the infection of at least a component of white blood cells by CHIKV, and shows that CHIKV infection results in activation of the inflammasome in a manner that is associated with disease severity.Publication Open Access The Cambodia Research Consortium: expediting research for malaria elimination with the emergency response to artemisinin resistance framework(2016) Canavati, Sara E.; Harriet L. S. Lawford; Fatunmbi, Bayo S.; Dysoley Lek; Rithea Leang; Samphor, Narann Top; Dondorp, Arjen M; Rekol Huy; Kazadi, Walter M.; Mahidol University. Faculty of Tropical Medicine. Department of Clinical Tropical MedicineThis commentary offers insight into how to best address barriers that may hinder the translation of malaria research findings into policy. It also proposes viable methods of implementing these policies in Cambodia. Currently, a wide range