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Item Metadata only Black ginger extract and its active compound, 5,7-dimethoxyflavone, increase intestinal drug absorption via efflux drug transporter inhibitions(2023-06-01) Boonnop R.; Meetam P.; Siangjong L.; Tuchinda P.; Thongphasuk P.; Soodvilai S.; Soodvilai S.; Mahidol University,7-dimethoxyflavone (DMF) on drug efflux mediated by breast cancer resistance proteins (BCRP) and P-glycoprotein (P-gp) in Caco-2 cells and heterologous cell systems [Madin-Darby canine kidney type II (MDCKII) stably transfected with human BCRP (MDCKII/BCRP) or human... or P-gp. The effect of the extract on 3H-Digoxin and 3H-Estrone sulfate transport was related to a decrease in efflux ratio. Likewise, DMF (5 μM) significantly increased 3H-Digoxin and 3H-Estrone sulfate absorption with a decreased efflux ratio comparedPublication Metadata only Novel chitosan-magnesium aluminum silicate nanocomposite film coatings for modified-release tablets(2011-04-04) Wanwisa Khunawattanakul; Satit Puttipipatkhachorn; Thomas Rades; Thaned Pongjanyakul; Khon Kaen University; Mahidol University; University of Otagoevaluated for use in tablet film coating. Effects of CS-MAS ratio and coating level on water uptake and drug release from the coated tablets were investigated. Surface and film matrix morphology of the coated film and the effect of enzymes in the simulated... gastro-intestinal fluid on drug release were also examined. The results demonstrated that the CS-MAS coated tablets had a rough surface and a layered matrix film, whereas a smooth surface and dense matrix film on the CS coated tablets was found. HoweverPublication Metadata only Characterization and in vitro evaluation of intestinal absorption of liposomes encapsulating zanamivir(2011-07-01) Boontarika Boonyapiwat; Narong Sarisuta; Sarinnate Kunastitchai; Thailand Ministry of Public Health; Mahidol UniversityZanamivir is currently used for the treatment of H1N1 and H5N1 influenza viruses. Due to its highly hydro-philic property, zanamivir has poor oral bioavailability. Liposomal formulations are known to improve oral absorption of hydrophilic drugs.... The present study investigates the effect of liposomes encapsulating zanamivir on the permeation of zanamivir across Caco-2 monolayers. Among the formulations studied, neutral liposomes composed of Phospholipon ® 90 G and cholesterol at molar ratio of 7:3Publication Metadata only Inhibition of CFTR-mediated intestinal chloride secretion by a fungus-derived arthropsolide A: Mechanism of action and anti-diarrheal efficacy(2020-10-15) Nattaphong Akrimajirachoote; Saravut Satitsri; Ubonta Sommart; Vatcharin Rukachaisirikul; Chatchai Muanprasat; Rajabhat University; Mahidol University; Prince of Songkla University+ channels. Arthropsolide A had no effect on Na+-K+ ATPase activity. Interestingly, the inhibitory effect of arthropsolide A on CFTR was attenuated by cell depolarization and AMPK inhibition independent of multi-drug resistance protein 4, phosphodiesterases... fluid loss in secretory diarrheas. This study aimed to identify novel CFTR inhibitors from fungal metabolites and explore its underlying mechanisms and potential utility in secretory diarrheas. Electrophysiological analyses in human intestinal epithelialItem Metadata only AMPK in Intestinal Health and Disease: A Multifaceted Therapeutic Target for Metabolic and Inflammatory Disorders(2025-01-01) Yibcharoenporn C.; Muanprasat C.; Moonwiriyakit A.; Satitsri S.; Pathomthongtaweechai N.; Yibcharoenporn C.; Mahidol Universityregulator of cellular energy levels, 5’-adenosine monophosphate-activated protein kinase (AMPK) is essential for intestinal health. Beyond its established metabolic role, emerging evidence suggests that AMPK exerts profound effects on intestinal cell...The intestines play essential roles in nutrient absorption and immune function and help maintain a protective barrier. Disruptions to its function can result in various diseases, including metabolic disorders, inflammation, and cancer. As a keyItem Metadata only Myosin light chain kinase-mediated epithelial barrier dysfunction as a potential pathogenic mechanism of afatinib-induced diarrheas: A study in human colonoid model(2025-01-15) Worakajit N.; Satitsri S.; Kitiyakara T.; Muanprasat C.; Worakajit N.; Mahidol UniversityDiarrheas are an important adverse effect of afatinib, a tyrosine kinase inhibitor (TKI) anti-cancer drug, leading to mortality and morbidity in cancer patients with their pathophysiological mechanisms related to intestinal barrier dysfunctions... being poorly understood. This study aimed to investigate the effect of afatinib on intestinal epithelial barrier integrity using a human colon-derived organoid model (colonoids). Afatinib (0.5 μM) significantly decreased the transepithelial electricalPublication Metadata only In vitro intestinal transport of phyllanthin across Caco-2 cell monolayers(2013-01-01) D. H. Nguyen; N. Sinchaipanid; A. Mitrevej; Mahidol Universitythe processes of intestinal absorption and transport mechanisms of phyllanthin. Phyllanthin was isolated from Phyllanthus amarus leaves and purified to obtain a purity of more than 98%. Caco-2 cell monolayers were used as an in vitro model of the human... intestinal barrier. Bidirectional studies of phyllanthin across Caco-2 cell monolayers were performed to investigate the roles of active and passive mechanisms of phyllanthin intestinal transport. The transport mechanism of phyllanthin across Caco-2 cellPublication Metadata only Alginate-magnesium aluminum silicate films: Effect of plasticizers on film properties, drug permeation and drug release from coated tablets(2007-03-21) Thaned Pongjanyakul; Satit Puttipipatkhachorn; Khon Kaen University; Mahidol Universityof the SA-MAS films and the plasticized films could control the drug release from tablets in gastro-intestinal condition. © 2006 Elsevier B.V. All rights reserved....The effect of hydrophilic plasticizers, namely glycerin and polyethylene glycol 400 (PEG400), on physicochemical properties of sodium alginate-magnesium aluminum silicate (SA-MAS) microcomposite films was characterized and application of the filmsPublication Metadata only Identification of new small molecule inhibitors of cystic fibrosis transmembrane conductance regulator protein: In vitro and in vivo studies(2007-03-01) Chatchai Muanprasat; Santi Kaewmokul; Varanuj Chatsudthipong; Mahidol University-induced intestinal fluid secretion by 40%, whereas INH 2 produced no effect. Our results indicate that INH 1 could be a new class candidate for a blocker of cholera toxin-induced intestinal fluid secretion as well as a CFTR inhibitor. © 2007 Pharmaceutical...Cystic fibrosis transmembrane conductance regulator (CFTR) protein is a cAMP-regulated chloride channel that has been proposed as a pharmacological target to reduce intestinal fluid loss in cholera. The aim of this study was to identify new CFTRItem Metadata only Potential lipid-lowering effects of preussin B on inhibition of intestinal cholesterol absorption: integrative mechanisms of action and proteomic analysis(2025-01-01) Ontawong A.; Inchai J.; Holasut P.; Chiangjong W.; Chieochansin T.; Crucitti D.; Orgueira A.M.; Rojanaverawong W.; Saithong T.; Tunkaew K.; Wiriyawaree H.; Thongpat K.; Tadpetch K.; Rukachaisirikul V.; Vaddhanaphuti C.S.; Ontawong A.; Mahidol Universityeffects by inhibiting intestinal cholesterol absorption in human colorectal adenocarcinoma (Caco-2) cells and in an ex vivo intestinal loop in rats, comparable to those of ezetimibe. Ezetimibe is the only drug that targets reducing intestinal cholesterol...Introduction: Hyperlipidemia remains a significant risk factor for cardiovascular diseases and is a leading cause of death, driving the need for novel and effective cholesterol-lowering agents. Preussin (1) has recently demonstrated lipid-lowering