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Item Open Access Synthetic strategies for the preparation of affinity label dynorphin A(1-11) NH(2) analogues(1997) Leena Leelasvatanakij; Aldrich, J.V.Publication Open Access Solid phase synthesis of affinity label containing dynorphin a analogues assembled on PAL-PEG PS vs PAL-PS resins(1996) Leena Leelasvatanakij; ลีณา ลีลาศวัฒนกิจ; Aldrich Jane V.; เจน วี อัลดริชPublication Metadata only A solid-phase synthetic strategy for the preparation of peptide-based affinity labels: Synthesis of dynorphin A analogs(2000-08-19) L. Leelasvatanakij; Jane V. Aldrich; University of Maryland, Baltimore; Mahidol UniversitySolid-phase synthetic methodology was developed for the preparation of peptide-based affinity labels. The initial peptides synthesized were dynorphin A (Dyn A) analogs, [Phe(p-X)4,D-Pro10]Dyn A(1-11)NH2 containing isothiocyanate (X=-N=C... glycol-polystyrene (PEG-PS) resins containing the PAL [peptide amide linker, 5-(4-Fmoc-aminomethyl-3,5-dimethoxyphenoxy)valeric acid] linker. Both the rate of Alloc deprotection and the purity of the crude affinity-labeled peptides obtained were foundPublication Metadata only Hatching envelope formation in the egg of the black tiger shrimp, Penaeus monodon (Decapoda, Penaeidae)(2013-08-01) Pattira Pongtippatee; Wanita Putthawat; Pornsawan Dungsuwan; Wattana Weerachartyanukul; Boonsirm Withyachumnarnkul; Prince of Songkla University; Mahidol University; National Center for Genetic Engineering and Biotechnology (BIOTEC)The aim of this study was to reveal the process of hatching envelope (HE) formation in eggs of the black tiger shrimp Penaeus monodon, using fluorocytochemistry with fluorescein isothiocyanate (FITC)-labelled lectins and transmission electron... microscopy (TEM) with mouse monoclonal anti-FITC-conjugated gold-lectin labelling. Following lectin binding screening tests, Concanavalin A (Con A) and wheat germ agglutinin (WGA) were chosen to trace movements of specific sugar-associated componentsPublication Metadata only Reduction - Mediated 99mTc - Labeling of Antitumor Monoclonal Antibodies : Effect of Increasing Specific Activity on Antibody Binding Kinetics(1998-10-01) Vipa Boonkitticharoen; Duangpen Puchinda; Puthiporn Chouplywech; Wirawat Jalayondeja; Mahidol UniversityReduction-mediated 99mTc-labeling of antibodies has gained widespread acceptance in preparation of tumor imaging agents. Increased specific activity to enhance detection signals has raised the question of whether such an attempt would cause change... in antibody binding kinetics. To answer this question, two antitumor monoclonal antibodies, i.e. IOR-CEA (IgG1) and EMD (IgG2a) were labeled with 99mTc to yield specific activities ranging from 549-4414 MBq/mg. Regression analysis of the binding data revealedPublication Metadata only Label-free guided mode resonance sensor for detection of glycated hemoglobin(2017-11-22) Boonrasri Seeleang; Chamras Promptmas; Sakoolkan Boonruang; Waleed S. Mohammed; Romuald Jolivot; Mahidol University; Thailand National Electronics and Computer Technology Center; Bangkok Universitylevel in diabetes patients. Here, the sensing approach utilizes boronate affinity by modification of the GMR surface with 3- Aminophenylboronic acid (PBA). The sensing signal is then measured via a resonance shift in a reflection spectrum as the changePublication Metadata only Immunoelectron microscopic localization of laminin in rat ovarian follicles(1992-01-01) Vijittra Leardkamolkarn; Dale R. Abrahamson; Mahidol University; University of Alabama at BirminghamWe studied the immunohistochemical and ultrastrural distribution of laminin in ovaries of immature and mature rats. When sections from 1–8‐week‐old rat ovaries were labeled directly with conjugates of affinity purified anti‐laminin IgG‐horseradish... peroxidase (HRP), the antibodies bound to all ovarian basement membranes including those surrounding follicles in different stages of maturation. In addition, intracellular labeling was seen in granulosa and theca cells of follicles undergoing rapidPublication Metadata only Novel paper-based colorimetric immunoassay (PCI) for sensitive and specific detection of salbutamol residues in flesh of swine and urine using Ag3PO4/Ag nanocomposite as label(2020-01-01) Nutthaporn Malahom; Purim Jarujamrus; Wipark Anutrasakda; Runglawan Chawengkirttikul; Atitaya Siripinyanond; Rattapol Meelapsom; Maliwan Amatatongchai; Ubon Rajathanee University; Chulalongkorn University; Mahidol University; Kalasin Universitycolorimetric immunoassay (PCI) using the Ag3PO4/Ag nanocomposite as label for sensitive and specific determination of SAL in flesh of swine and urine. The Ag3PO4/Ag nanocomposite was synthesized by a one-step chemical bath method, which could instantly oxidize... a chromogenic substrate for the color development under acidic conditions without the participation of H2O2. This approach provides high affinity between the Ag3PO4/Ag nanocomposite and the substrate (with the Michaelis–Menten constant of 0.44 mItem Metadata only Cyclodextrin-integrated molecularly imprinted polymer-based QCM sensor for sensitive, label-free detection of Gal3(2025-11-01) Longsompurana P.; Wolschann P.; Subannajui K.; Lieberzeit P.A.; Poo-arporn R.P.; Longsompurana P.; Mahidol Universityand morbidity associated with HF, early diagnosis is critical, necessitating a rapid, sensitive, and straightforward method for Gal3 detection. This study presents an innovative, label-free quartz crystal microbalance (QCM) sensor designed for Gal3 detection... dissociation constant (KD) of 2.03 nM. This indicates a stronger binding affinity compared to the non-molecularly imprinted polymer (NIP), which exhibited a KD of 26.75 nM. The sensor exhibits a linear detection range of 1–30 ng/mL, with aPublication Metadata only Analysis of saturation binding and saturation infection for dengue serotypes 1 and 2 in liver cells(2003-02-15) Lukkana Suksanpaisan; Duncan R. Smith; Mahidol Universitytherefore sought to investigate the binding of the dengue virus to HepG2 cells. Methods: Radio-labeled dengue virus serotypes 1 and 2 were prepared through viral propagation in Vero cells. Increasing amounts of virus were then incubated with HepG2 cells... serotype 2 became saturated at levels of approximately 3,000 viruses per cell. Conclusions: These results are consistent with proposals that dengue virus binding to cells is mediated initially through a low-affinity interaction with an abundant molecule