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Item Metadata only Rapid diagnosis of bacteremia and anaerobic infection by gas liquid chromatography(Mahidol University. Mahidol University Library and Knowledge Center, 2024) Somchai Santiwatanakul; Malai VorachitPublication Metadata only Prognostic value of serum procalcitonin level for the diagnosis of bacterial infections in critically-ill patients(2019-01-01) Apichot So-Ngern; Sombat Leelasupasri; Suvatna Chulavatnatol; Chalermsri Pummangura; Pakwan Bunupuradah; Preecha Montakantikul; Siam University; Faculty of Medicine, Ramathibodi Hospital, Mahidol University; Mahidol University; Phyathai 2 International Hospital-ill patients. However, the cut-off value of PCT for the diagnosis of bacterial infections is unclear and unreliable. This study aimed to determine the optimal cut-off value of PCT for the diagnosis of bacterial infections in critically-ill patients... predictive value of 83.7%. Conclusion: PCT was found to be an accurate biomarker for the diagnosis of bacterial infections among patients admitted to medical and surgical intensive care units. The optimal cut-off value of PCT for the diagnosis of bacterialPublication Metadata only Identification of circulating bacterial antigens by in vivo microbial antigen discovery(2011-07-01) Dana E. Nuti; Reva B. Crump; Farida Dwi Handayani; Narisara Chantratita; Sharon J. Peacock; Richard Bowen; Philip L. Felgner; D. Huw Davies; Terry Wu; C. Rick Lyons; Paul J. Brett; Mary N. Burtnick; Thomas R. Kozel; David P. AuCoina; University of Nevada School of Medicine; Mahidol University; University of Cambridge; Colorado State University; University of California, Irvine; University of New Mexico Health Sciences Center; University of South AlabamaDetection of microbial antigens in clinical samples can lead to rapid diagnosis of an infection and administration of appropriate therapeutics. A major barrier in diagnostics development is determining which of the potentially hundreds or thousands... to identify circulating bacterial antigens. This technique starts with "InMAD serum," which is filtered serum that has been harvested from BALB/c mice infected with a bacterial pathogen. The InMAD serum, which is free of whole bacterial cells, is usedPublication Metadata only The use of dried cerebrospinal fluid filter paper spots as a substrate for PCR diagnosis of the aetiology of bacterial meningitis in the Lao PDR(2013-01-01) I. Elliott; S. Dittrich; D. Paris; A. Sengduanphachanh; P. Phoumin; P. N. Newton; Mahosot Hospital; Nuffield Department of Clinical Medicine; Mahidol UniversityWe investigated whether dried cerebrospinal fluid (CSF) conserved on filter paper can be used as a substrate for accurate PCR diagnosis of important causes of bacterial meningitis in the Lao PDR. Using mock CSF, we investigated and optimized filter... potentially help us to better understand the epidemiology of bacterial meningitis in resource-poor settings and guide empirical treatments and vaccination policies. © 2013 The Authors. Clinical Microbiology and Infection published by John Wiley & Sons LtdPublication Open Access Performance of C-reactive protein and procalcitonin to distinguish viral from bacterial and malarial causes of fever in Southeast Asia(2015) Yoel Lubell; Blacksell, Stuart D.; Susanna Dunachie; Ampai Tanganuchitcharnchai; Thomas Althaus; Wanitda Watthanaworawit; Paris, Daniel H.; Mayfong Mayxay; Peto, Thomas J.; Dondorp, Arjen M.; White, Nicholas J.; Day, Nicholas P.J.; François Nosten; Newton, Paul N.; Paul Turner; Mahidol University. Faculty of Tropical Medicine. Mahidol-Oxford Tropical Medicine Research Unit (MORU), Laos and, Thailand. Of the 1372 patients with a microbiologically confirmed diagnosis, 1105 had a single viral, bacterial or malarial infection. Procalcitonin and CRP levels were compared amongst these aetiological groups and their sensitivity..., there are no similar tests to guide the use of antibiotics in undifferentiated fevers. In this study we estimate the diagnostic accuracy of two well established biomarkers of bacterial infection, procalcitonin and Creactive protein (CRP) in discriminating betweenItem Metadata only Rapid diagnosis of intra-amniotic infection using nanopore-based sequencing(2022-01-01) Chaemsaithong P.; Romero R.; Pongchaikul P.; Vivithanaporn P.; Lertrut W.; Jaovisidha A.; Mongkolsuk P.; Nitayanon P.; Pongsuktavorn K.; Kamlungkuea T.; Jung E.; Suksai M.; Singhsnaeh A.; Jenjaroenpun P.; Thaipisuttikul I.; Wongsurawat T.; Mahidol Universityat the bacterial species level was achieved within 5-9 h from DNA extraction. This is the first study demonstrating that the nanopore sequencing technique is capable of rapid diagnosis of intra-amniotic infection using fresh amniotic fluid samples....Early diagnosis and treatment of intra-amniotic infection is crucial. Rapid pathogen identification allows for a definite diagnosis and enables proper management. We determined whether the 16S amplicon sequencing performed by a nanopore sequencingPublication Metadata only Correlation of roentgenologic diagnosis and etiologic agents of pneumonia in thai children under 5 years of age.(1993-10-01) R. Pornkul; S. Suwanjutha; S. Ruangkanchanasetr; Mahidol Universitybe usually be differentiated easily from that of bacterial pneumonia. Percentage of agreement of roentgenographic diagnosis for bacterial pneumonia, viral pneumonia and mixed infection is about 53, 73.3 and 63.9 per cent respectively....An attempt to distinguish between viral and bacterial pneumonia from radiographs is usually difficult unless there is a pathognomonic type of infiltration such as parahilar peribronchial infiltration commonly seen in viral pneumonia which canPublication Metadata only The diagnostic utility of procalcitonin for the detection of infection among systemic lupus erythematosus patients in a tertiary care hospital(2021-06-01) Praparat Keochompoo; Sathien Sukpanichnant; Ajchara Koolvisoot; Susan Assanasen; Siriraj Hospital; Buddhachinaraj Hospital.04 to 0.16), p<0.001. The cut-off point of elevated serum PCT (0.5 ng/mL) for the diagnosis of bacterial or fungal infections in patients with SLE had sensitivity, specificity, positive predictive value, and negative predictive values of 51.3%, 95.3%, 83...Background: Clinical manifestations of infection in patients with systemic lupus erythematosus (SLE) are variable and involve significant diagnostic challenges. Delays in diagnosis and treatment strongly affect the clinical outcomes and mortalityItem Metadata only Diagnostic Yield and Impact on Antimicrobial Management of 16S rRNA Testing of Clinical Specimens(2022-11-01) Eamsakulrat P.; Santanirand P.; Phuphuakrat A.; Mahidol Universityand December 2021 in a university hospital were prospectively included in this study. A total of 434 specimens from 374 patients were requested. Of these, 253 (58.3%) specimens were collected from patients whose final diagnosis indicated a bacterial infection..., whereas 181 (41.7%) specimens were from nonbacterial infections. Using the final diagnosis as a “gold standard,” the sensitivity and specificity of 16S rRNA gene sequencing were 38.3% and 93.9%, respectively. Among the bacterial infection casesPublication Metadata only Adapting microarray gene expression signatures for early melioidosis diagnosis(2020-07-01) Ornuma Sangwichian; Toni Whistler; Arnone Nithichanon; Chidchamai Kewcharoenwong; Myint Myint Sein; Chawitar Arayanuphum; Narisara Chantratita; Ganjana Lertmemongkolchai; Centers for Disease Control and Prevention; Khon Kaen University; Thailand Ministry of Public Health; Mahidol Universityresult in misdiagnosis, failure of treatment, and poor clinical outcome. Septicemia with septic shock is the most common cause of death, with mortality rates above 40%. Bacterial culture is the gold standard for diagnosis, but it has low sensitivity... and 29 patients with other bacterial infections to validate the test and determine cutoff values for use in a cascading diagnostic algorithm. Differentiation of septicemic melioidosis from other sepsis cases had a sensitivity of 82%, specificity of 93