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Item Metadata only Development of a non radioactive DNA probe method for the diagnosis of baculovirus in the Black Tiger Prawn(Mahidol University. Mahidol University Library and Knowledge Center, 1997) Kanchana Dokladda; Pichit Tosukhowong; Prayad KomaratatPublication Metadata only Production of Japanese encephalitis virus-like particles using the baculovirus-insect cell system(2012-12-01) Hideki Yamaji; Maiko Segawa; Masataka Nakamura; Tomohisa Katsuda; Miwa Kuwahara; Eiji Konishi; Kobe University; Kobe University School of Medicine; Mahidol UniversityThe production of a secreted form of Japanese encephalitis (JE) virus-like particles (VLPs) using the baculovirus-insect cell system was investigated. A recombinant baculovirus that contained the JE virus (JEV) prM signal sequence and the genes... with the recombinant baculovirus had secreted the E protein. Sucrose density-gradient sedimentation analysis of the culture supernatant suggested that secreted E antigen molecules were in a particulate form. Baculovirus-infected Sf9 cells produced more than a 10-foldItem Metadata only Expression of recombinant NS1 protein in baculovirus system and development of monoclonal antibody against NS1 protein of avian influenza a virus (H5N1)(Mahidol University. Mahidol University Library and Knowledge Center, 2010) Patumporn Jiean-Umpunkul; Tararaj Dharakul; Kanokwan PoomputsaPublication Metadata only Recombinant baculovirus mediates dsRNA specific to rr2 delivery and its protective efficacy against WSSV infection(2016-07-10) Triwit Rattanarojpong; Suthiwat Khankaew; Pongsak Khunrae; Rapeepun Vanichviriyakit; Kanokwan Poomputsa; King Mongkut s University of Technology Thonburi; Mahidol Universityto develop dsRNA expression in shrimp for control of WSSV infection, we developed a recombinant baculovirus expressing recombinant VP28 as the gene delivery system to carry a gene encoding dsRNA specific to rr2 for triggering the RNAi process in shrimp.... The results showed that the recombinant baculovirus harboring VP28 was able to express VP28 indicated by Western blot with polyclonal antibody specific to VP28. VP28 transcript was detected in shrimp hemocytes after co-culture hemocytes with the recombinantPublication Metadata only Expression of human pyruvate carboxylase in insect cells using the baculovirus system(2004-03-26) Natthida Petchampai; Vichai Boonsaeng; John C. Wallace; Sarawut Jitrapakdee; Mahidol University; University of Adelaide-transfected with the baculovirus genome into Sf9 cells to produce recombinant baculoviruses harbouring human PC cDNA. The expression of human PC under the control of the polyhedrin promoter was found to be at its highest level at 4 days post-infectionPublication Metadata only Monodon baculovirus (MBV) infects the freshwater prawn Macrobrachium rosenbergii cultivated in Thailand(2010-03-01) Warachin Gangnonngiw; Kesinee Laisutisan; Siriporn Sriurairatana; Saengchan Senapin; Niti Chuchird; Chalor Limsuwan; Parin Chaivisuthangkura; Timothy W. Flegel; Mahidol University; Thailand National Science and Technology Development Agency; Kasetsart University; Srinakharinwirot University. These inclusions resembled those produced by some baculoviruses prior to formation of occlusion bodies that enclose virions in a polyhedrin protein matrix. By electron microscopy, the intranuclear inclusions contained bacilliform, enveloped virions (approximately... of approximately 20 nm diameter resembling polyhedrin subunits of occlusion bodies (OB) of a bacilliform virus of the black tiger shrimp Penaeus monodon, previously reported from Thailand and called monodon baculovirus (MBV). It is currently listedPublication Metadata only Characterization of Anopheles minimus CYP6AA3 expressed in a recombinant baculovirus system(2008-09-01) Soamrutai Boonsuepsakul; Ekawan Luepromchai; Pornpimol Rongnoparut; Mahidol University; Chulalongkorn Universityminimus mosquito, a major malaria vector in Thailand. In the present study, we further investigated the role of CYP6AA3 enzyme in deltamethrin metabolism in vitro. The CYP6AA3 was expressed in Spodoptero frugiperda (Sf9) insect cells via baculovirusPublication Metadata only Molecular cloning of a Plasmodium falciparum gene interrupted by 15 introns encoding a functional primase 53 kDa subunit as demonstrated by expression in a baculovirus system(1996-11-28) Suttiphan Prasartkaew; Natasha M. Zijlstra; Prapon Wilairat; J. Prosper Overdulve; Erik De Vries; Utrecht University; Mahidol Universitygene was expressed as a fusion product containing an N-terminal polyhistidine tag using a baculovirus expression vector. The protein was overproduced in insect cells and purified. Activity assays demonstrated the ability of the p53 subunit to initiatePublication Metadata only Production of Japanese encephalitis virus-like particles in insect cells(2013-11-19) Hideki Yamaji; Eiji Konishi; Kobe University; Mahidol UniversityVirus-like particles (VLPs) are composed of one or several recombinant viral surface proteins that spontaneously assemble into particulate structures without the incorporation of virus DNA or rna. The baculovirus-insect cell system has been used... extensively for the production of recombinant virus proteins including VLPs. While the baculovirus-insect cell system directs the transient expression of recombinant proteins in a batch culture, stably transformed insect cells allow constitutive productionPublication Metadata only An evidence on trans-ovarian transmission of Monodon baculovirus (MBV) infection in Penaeus monodon(2015-06-01) Duangkhaetita Kanjanasopa; Pattira Pongtippatee; Rapeepun Vanichviriyakit; Somjai Wongtripop; Padmaja Jayaprasad Pradeep; Boonsirm Withyachumnarnkul; Prince of Songkla University; Mahidol University; Shrimp Genetic Improvement Center© 2015 Elsevier B.V. Monodon baculovirus (MBV) causes slow growth of the shrimp being due to its infection in the hepatopancreas, the organ producing digestive enzymes and for nutrient storage. It has long been documented that the virus