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Publication Open Access Evaluation of a latex agglutination assay for the identification of Burkholderia pseudomallei and Burkholderia mallei.(2014-04-07) Duval, Brea D.; Elrod, Mindy G.; Gee, Jay E.; Narisara Chantratita; นริศรา จันทราทิตย์; Sarunporn Tandhavanant; ศรัณย์พร ตันฑวนันท์; Direk Limmathurotsakul; ดิเรก ลิ้มมธุรสกุล; Hoffmaster, Alex R.; Hoffmaster, Alex R.; Mahidol University. Faculty of Tropical Medicine. Department of Microbiology and Immunology.; Mahidol University. Faculty of Tropical Medicine. Mahidol-Oxford Tropical Medicine Research Unit.; Mahidol University. Faculty of Tropical Medicine.Department of Tropical Hygiene.Cases of melioidosis and glanders are rare in the United States, but the etiologic agents of each disease (Burkholderia pseudomallei and Burkholderia mallei, respectively) are classified as Tier 1 select agents because of concerns about.... mallei isolates. This assay uses a monoclonal antibody that specifically recognizes the capsular polysaccharide produced by B. pseudomallei and B. mallei, but is absent in closely related Burkholderia species. A total of 110 B. pseudomallei and BPublication Metadata only Antigenic relatedness between Burkholderia pseudomallei and Burkholderia mallei(2002-01-01) Narisara Anuntagool; Stitaya Sirisinha; Mahidol UniversityBurkholderia pseudomallei and Burkholderia mallei are causative agents of distinct diseases, namely, melioidosis and glanders, respectively. The two species are very closely related, based on DNA-DNA homology, base sequence of the 16S r...RNA, and phenotypic characteristics. Based on the use of polyclonal antisera, B. pseudomallei and B. mallei are also found to be antigenically closely related to one another. We previously reported the production of monoclonal antibodies (MAbs) against B. pseudomalleiPublication Metadata only Short report: Evaluation of a latex agglutination assay for the identification of Burkholderia pseudomallei and Burkholderia mallei(2014-01-01) Brea D. Duval; Mindy G. Elrod; Jay E. Gee; Narisara Chantratita; Sarunporn Tandhavanant; Direk Limmathurotsakul; Alex R. Hoffmaster; Centers for Disease Control and Prevention; Mahidol UniversityCases of melioidosis and glanders are rare in the United States, but the etiologic agents of each disease (Burkholderia pseudomallei and Burkholderia mallei, respectively) are classified as Tier 1 select agents because of concerns about.... mallei isolates. This assay uses a monoclonal antibody that specifically recognizes the capsular polysaccharide produced by B. pseudomallei and B. mallei, but is absent in closely related Burkholderia species. A total of 110 B. pseudomallei and B. malleiPublication Metadata only PCR-RFLP based differentiation of Burkholderia mallei and Burkholderia pseudomallei(2004-04-01) Suda Tanpiboonsak; Atchara Paemanee; Sasinee Bunyarataphan; Sumalee Tungpradabkul; Mahidol UniversityBurkholderia mallei and Burkholderia pseudomallei manifest a high similarity with regard to clinical syndromes, glanders and melioidosis. Phenotypic and genotypic characters are also highly similar. In an attempt to differentiate the two organisms..., the molecular method was applied. This study aimed to identify the different DNA fragment in B. mallei, as compared with B. pseudomallei. The Sau3AI-digested genomic DNA patterns of B. mallei and B. pseudomallei are distinctive, especially the DNA fragmentsItem Metadata only Regulation of a flagellin gene in Burkholderia mallei (a non-motile species)(Mahidol University. Mahidol University Library and Knowledge Center, 2000) Atchara Paemanee; Sumalee Tungpradabkul; Wilai NoonpakdeePublication Metadata only Antibodies from patients with melioidosis recognize Burkholderia mallei but not Burkholderia thailandensis antigens in the indirect hemagglutination assay(2005-09-01) Rachaneeporn Tiyawisutsri; Sharon J. Peacock; Sayan Langa; Direk Limmathurotsakul; Allen C. Cheng; Wirongrong Chierakul; Wipada Chaowagul; Nicholas P.J. Day; Vanaporn Wuthiekanun; Mahidol University; Chulalongkorn University; Nuffield Department of Clinical Medicine; Menzies School of Health Research; Geelong Hospital; Sappasitthiprasong HospitalThe indirect hemagglutination assay routinely used to detect antibodies to Burkholderia pseudomallei was modified to detect cross-reactivity of antibodies to B. pseudomallei, B. mallei, and B. thailandensis antigens. We demonstrate a lack of cross...-reactivity between B. pseudomallei and B. thailandensis but marked cross-reactivity between B. pseudomallei and B. mallei. Copyright © 2005, American Society for Microbiology. All Rights Reserved.Publication Metadata only Antimicrobial drug-selection markers for Burkholderia pseudomallei and B. mallei(2008-11-01) Herbert P. Schweizer; Sharon J. Peacock; Colorado State University; Mahidol University; University of OxfordGenetic research into the select agents Burkholderia pseudomallei and B. mallei is currently hampered by a paucity of approved antimicrobial drug-selection markers. The strict regulations imposed on researchers in the United States but not in otherPublication Metadata only Short report: Pulsed-field gel electrophoresis as a discriminatory typing technique for the biothreat agent Burkholderia mallei(2006-03-01) Narisara Chantratita; Mongkol Vesaratchavest; Vanaporn Wuthiekanun; Rachaneeporn Tiyawisutsri; Tsedev Ulziitogtokh; Erhan Akcay; Nicholas P J Day; Sharon J. Peacock; Mahidol University; Biocombinat; Central Veterinary Control and Research InstitutePulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) was used to type 21 laboratory strains of Burkholderia mallei. We demonstrated good resolution by PFGE together with clustering of some geographically related isolates..., and confirmed previous observations that B. mallei is clonal as defined by MLST. Copyright © 2006 by The American Society of Tropical Medicine and Hygiene.Publication Metadata only Melioidosis patient serum-reactive synthetic tetrasaccharides bearing the predominant epitopes of Burkholderia pseudomallei and Burkholderia mallei O-antigens(2019-01-01) Maude Cloutier; Emmanilo Delar; Kevin Muru; Seynabou Ndong; Robert R. Hoyeck; Taniya Kaewarpai; Narisara Chantratita; Mary N. Burtnick; Paul J. Brett; Charles Gauthier; INRS-Institut Armand Frappier; Mahidol University; University of Nevada School of Medicine© 2019 The Royal Society of Chemistry. Melioidosis and glanders, respectively caused by the Gram-negative bacteria Burkholderia pseudomallei (Bp) and Burkholderia mallei (Bm), are considered as urgent public health issues in developing countriesPublication Metadata only Burkholderia Hep_Hap autotransporter (BuHA) proteins elicit a strong antibody response during experimental glanders but not human melioidosis(2007-04-11) Rachaneeporn Tiyawisutsri; Matthew T.G. Holden; Sarinna Tumapa; Sirirat Rengpipat; Simon R. Clarke; Simon J. Foster; William C. Nierman; Nicholas P.J. Day; Sharon J. Peacock; Mahidol University; Chulalongkorn University; Wellcome Trust Sanger Institute; University of Sheffield; J. Craig Venter Institute; George Washington University School of Medicine and Health Sciences; Nuffield Department of Clinical MedicineBackground. The bacterial biothreat agents Burkholderia mallei and Burkholderia pseudomallei are the cause of glanders and melioidosis, respectively. Genomic and epidemiological studies have shown that B. mallei is a recently emerged, host... architectures with hemagglutinins and invasins. These have been designated Burkholderia Hep_Hag autotransporter (BuHA) proteins. A total of 110/207 positive clones (53%) of a B. mallei expression library screened with sera from two infected horses belonged